Core Facilities Categories

Contact: Nancy Breen

Gas Chromatograph - Mass Spectrometer (GC - MS)

Agilent 5890 Gas Chromatograph - Mass Spectrometer (GC - MS) wth Mass Selective Detetor (MSD)
GC MS for teaching and research

Contact: Nancy Breen

High Performance Liquid Chromatography (HPLC)

Waters 2695 High Performance Liquid Chromatograph-MS / ZQ Micromass Mass Spectrometer
LC - MS for teaching and research

URI Sources, Transport, Exposure & Effects of PFAS (STEEP)

Contact: Nancy Breen

Gas Chromatograph

Agilent 7890 Gas Chromatography with DB-23 column
GC for fatty acid analysis

Facility	Instrument/Service/Resource Unit
URI Sources, Transport, Exposure & Effects of PFAS (STEEP) Contact: Simon Vojta	Mass Spectrometer SCIEX X500R QTOF PLATFORM coupled to an EXION LC AC SYSTEM A tandem (MS/MS) mass spectrometer combining a quadrupole and a time-of-flight analyzer allows collecting high-resolution MS and MRM spectra. The instrument is suitable for both quantitative analysis as well as non-targeted approach and operates under SCIEX OS software. The collected data are evaluated using an independent processing station (SCIEX Alpha Workstation 2020) equipped with SCIEX OS-Q software

Facility

Instrument/Service/Resource Unit

Contact: Simon Vojta

Mass Spectrometer

SCIEX X500R QTOF PLATFORM coupled to an EXION LC AC SYSTEM
A tandem (MS/MS) mass spectrometer combining a quadrupole and a time-of-flight analyzer allows collecting high-resolution MS and MRM spectra. The instrument is suitable for both quantitative analysis as well as non-targeted approach and operates under SCIEX OS software. The collected data are evaluated using an independent processing station (SCIEX Alpha Workstation 2020) equipped with SCIEX OS-Q software

Clinical Translational Research Resources

Facility	Instrument/Service/Resource Unit
Advance-CTR Contact: Heather Fournier	Research - Biostatistical Consulting, Epidemiology & Clinical Research Design Services Clinical Research Service, Advance-CTR A team of experienced statisticians and methodological experts are on-hand to help investigators prepare their proposals pre-award, and plan their projects upon receipt of funding. Pre-award services include, positioning of specific aims and hypotheses, measure selection, power analysis and data analysis planning. Post-award services include decision support services, data acquisition and management and consultations to assess the impact of potential design changes on final statistical influence. Data analysis and results interpretation services include implementing data analysis plans, statistical analysis consultations, selecting and accessing statistical analysis software, and coaching to best utilize statistical analysis software. Manuscript and grant proposal writing services include assistance with write-ups, data visualizations, integration of interpretations in the broader context of the project, and addressing comments from peer reviewers.
Advance-CTR Contact: Heather Fournier	Research - Biomedical Informatics Services Clinical Research Service, Advance-CTR Researchers may request, access, and use data available through the Unified Research data Sharing Access (URSA) infrastructure. The data include information originating from electronic health data systems at Lifespan, Care New England, and the Rhode Island Quality Institute, as well as from publicly accessible biological and health data sets.
IDeA COBRE Center for Addiction & Disease Risk Exacerbation (CADRE) Contact: Jennifer Tidey, Ph.D.	Clinical Laboratory Core Service The CADRE Clinical Laboratory Core (CLC) is a 'one-stop shopping' resource for clinical laboratory investigators. We provide laboratory space, supplies, equipment, research nursing capability, biomarker processing capability, scientific expertise and data management/analysis expertise. Provision of these resources via the CLC improves efficiency, consistency and economy of scale across projects.
Advance-CTR Contact: Heather Fournier	Research - Community Engagement & Outreach Core Clinical Research Service, Advance-CTR The CEO Core helps facilitate research endeavors that are relevant, accessible, and impactful to the entire state of Rhode Island.
Advance-CTR Contact: Heather Fournier	Administration Advance-CTR Clinical Research Service, Advance-CTR Service Coordination Advance-CTR works to support researchers at the state level by developing the infrastructure and resources investigators need to conduct clinical and translational research in Rhode Island. Through its interdisciplinary and inter-institutional model, Advance-CTR funds and supports research across the translational science spectrum, including basic science, clinical, and public health efforts. Ultimately, Advance-CTR seeks to connect researchers and support institutions in Rhode Island in order to advance clinical and translational research that improves public health in the state.
Advance-CTR Contact: Heather Fournier	Research - Qualitative Methods Services Clinical Research Service, Advance-CTR This service provides qualitative and mixed methods and data analysis design research support. Qualitative and mixed methods support includes; study design and preparation, original measure development, coaching on qualitative methods design decisions (focus groups, interviews, cognitive interviews), NVivo qualitative software training, help with grant writing of qualitative methods and analysis sections, manuscript and grant proposal review, post interview/focus group debriefing. Data analysis design support includes applied thematic analysis and framework matrix analysis.
Brown University Oncology Group (BRUOG) Contact: Howard Safran, MD	Research - Oncology Clinical Trials Support, Oncology Research The Brown University Oncology Research Group (BrUOG) mission is to improve cancer care through the implementation of innovative, multidisciplinary cancer clinical trials. BrUOG provides the infrastructure for the efficient development and implementation of these trials, which are created by Brown University faculty. BrUOG’s administrators and physicians provide support for the initial study concept and validation of trial design, and are responsible for trial administration, safety monitoring, data analysis, and the presentation and publication of findings. The trials sponsored by BrUOG investigate novel, cutting-edge applications of chemotherapy, biologic agents and other cancer treatments. Clinical trials are available for a broad range of disorders in hematology/oncology and these include treatment for cancers of the breast, brain, lung, gastrointestinal tract, skin, and prostate as well as trials in leukemia and lymphoma.
Brown-Lifespan Center for Digital Health Contact: Megan Ranney MD, MPH	Research - Emergency Digital Health Resources Clinical Research Resources, Emergency Digital Health EDHI’s mission is to use digital health to transform the care of patients with acute care needs before, during, and after their visit by developing and deploying effective, evidence-based digital health tools. The goal is to use technology to improve access to patients and increase the efficiency of health care delivery. As this field rapidly expands, it is critical for us to develop theoretically based interventions and to provide evidence that digital health tools are acceptable and effective, for both clinicians and patients. EDHI’s vision is for digital health to be an evidence-based, effective and well-integrated tool for creating and restoring health.
IDeA COBRE Center for Addiction & Disease Risk Exacerbation (CADRE) Contact: Jennifer Tidey, Ph.D.	IDeA COBRE Center for Addiction & Disease Risk Exacerbation (CADRE) Clinical Research Resources, Addiction & Disease Established in 2019, the Center for Addiction and Disease Risk Exacerbation (CADRE), is a federally funded NIH Center of Biomedical Research Excellence (COBRE). Through its affiliation with the Center for Alcohol and Addiction Studies at the Brown University School of Public Health, CADRE is investigating mechanisms whereby substance use impacts disease, using multi-disciplinary methods, including behavioral and physiological laboratory-based approaches across several substances of abuse.
IDeA COBRE Center for Addiction & Disease Risk Exacerbation (CADRE) Contact: Jennifer Tidey, Ph.D.	Analyzer - Automated Immunoassay System ProteinSimple - Ella Next Generation ELISA system The Ella mutiplex analyzer allows for multi-plexing of multiple biomarkers on the same cartridge. Rather than performing a separate ELISA for each biomarker with more sample volume to run in triplicate, the ELLA can multi-plex biomarkers in triplicate within a single well, increasing the amount of data that can be generated from clinical samples.
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	Design and production - COVID-19 Pseudovirus Service
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	Cell entry assays - COVID-19 Pseudovirus Service
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	SARS-CoV-2 spike and human ACE2 constructs Service

(Independent Nonprofit)

Facility	Instrument/Service/Resource Unit
Mansfield Bio-Incubator Contact: Alexander Margulis, PhD	Biological Safety Cabinet Labconco Purifier Logic Series Biological Safety Cabinet Provide personnel, product and environmental protection from hazardous particulates and agents that require biosafety level 1 or 2 containment.
Mansfield Bio-Incubator Contact: Alexander Margulis, PhD	Workstation - Laboratory and office space Mansfield Bio-Incubator Mansfield Bio-Incubator is a non-profit organization whose mission is to facilitate and assist the creation, growth, and success of the next generation of biotech companies. Our incubator assists these companies and nurtures entrepreneurial and economic development in the community by maintaining affordable lab and office space, services, training, mentoring, and a network of professional advisors. Shared and private office and lab space are available, with additional space coming online in 2021. More information on the membership rates can be found here. https://www.bioinc.org/membership-rates-2

Facility

Instrument/Service/Resource Unit

Contact: Alexander Margulis, PhD

Biological Safety Cabinet

Labconco Purifier Logic Series Biological Safety Cabinet
Provide personnel, product and environmental protection from hazardous particulates and agents that require biosafety level 1 or 2 containment.

Program for Environmental influences on Child Health Outcomes (ECHO)

Contact: Alexander Margulis, PhD

Workstation - Laboratory and office space

Mansfield Bio-Incubator
Mansfield Bio-Incubator is a non-profit organization whose mission is to facilitate and assist the creation, growth, and success of the next generation of biotech companies. Our incubator assists these companies and nurtures entrepreneurial and economic development in the community by maintaining affordable lab and office space, services, training, mentoring, and a network of professional advisors. Shared and private office and lab space are available, with additional space coming online in 2021. More information on the membership rates can be found here. https://www.bioinc.org/membership-rates-2

Facility	Instrument/Service/Resource Unit
Bradley Hasbro Children's Research Center Resources Contact: Gregory K. Fritz, MD	Research - Child Development and Mental Health Research Clinical Research Resources, Child Development The Bradley Hasbro Children's Research Center (BHCRC) encompasses a broad spectrum of research programs that share a commitment to studying the impact of psychological factors on the growth and development of children and their families. BHCRC investigators are exploring new insights into the genetic roots of autism; finding pediatric bio-behavioral markers of bipolar disorder; creating effective therapies for OCD; devising effective prevention strategies for adolescent sexual risk behaviors and obesity; and much more. Areas of interest include child and adolescent psychiatric disorders, forensic issues and juvenile justice, pediatric behavioral health, biological basis of psychiatric disorders, prevention and early intervention, and health services research.
Bradley Hasbro Children's Research Center Resources Contact: Gregory K. Fritz, MD	Research - Pediatric Mood, Imaging and Neurodevelopment (Pedi-MIND) program Clinical Research Service, Pediatric Imaging The goal of the Pediatric Mood, Imaging and Neurodevelopment (Pedi-MIND) program is to identify biological and behavioral markers of psychiatric illness in children and adolescents in order to improve diagnosis, treatment, and prevention of these conditions.
Bradley Hasbro Children's Research Center Resources Contact: Gregory K. Fritz, MD	Research - Rhode Island Consortium for Autism Research & Treatment (RI-CART) Clinical Research Resources, Autism The Rhode Island Consortium for Autism Research & Treatment (RI-CART) is working to improve the lives of Rhode Islanders with autism spectrum disorder and related neurodevelopmental disorders by promoting collaborative, cutting-edge research, improving health care and support services, and providing data-driven education and advocacy. We’re a community of families and individuals, researchers, clinicians, educators, and local agencies focused on both today’s and future generations.
Bradley Hasbro Children's Research Center Resources Contact: Gregory K. Fritz, MD	Research - Bradley Hospital Sleep and Chronobiology Research Laboratory (Sleep Lab) Clinical Research Resources, Sleep Sleep for Science is a program of research focusing on understanding how sleep patterns are shaped and how they affect other activities, functions, and biological processes. Our research is chiefly about sleep patterns and processes in young humans – children, teens, and young adults.
Bradley Hasbro Children's Research Center Resources Contact: Gregory K. Fritz, MD	Research - Developmental Disorders Genetics Research Clinical Research Resources, Developmental Genetics The Developmental Disorders Genetics Research Program was established in the fall of 2009 by Brown University to facilitate advances in translational research concerned with the genetics of developmental disorders.

(Lifespan - Hasbro Children's Hospital)

Facility	Instrument/Service/Resource Unit
Program for Environmental influences on Child Health Outcomes (ECHO) Contact: Phyllis Dennery, MD	Program for Environmental influences on Child Health Outcomes (ECHO) Clinical Research Resources, Environmental Influences on Child Health The ECHO program investigates how exposure to a range of environmental factors in early development — from conception through early childhood — influences the health of children and adolescents.

Facility

Instrument/Service/Resource Unit

Contact: Phyllis Dennery, MD

Program for Environmental influences on Child Health Outcomes (ECHO)

Clinical Research Resources, Environmental Influences on Child Health
The ECHO program investigates how exposure to a range of environmental factors in early development — from conception through early childhood — influences the health of children and adolescents.

Facility	Instrument/Service/Resource Unit
IDeA COBRE on Opioids and Overdose Contact: Heather Wunschel	Special Populations Core Service The Special Populations Core (SPC) provides infrastructure to recruit and retain vulnerable and underserved individuals, builds capacity in the ethical conduct of research with vulnerable populations, and serves as a centralized resource for training and outreach regarding the conduct of research among individuals most impacted by overdose and opioid-related harms.
Emergency Medicine Research Laboratory Contact: Gregory Jay	Research - Emergency Medicine Research Laboratory Resources Clinical Trials Support, Emergency Medicine The research program in the Department of Emergency was established to support investigator-initiated research and pharmaceutical, biologic and biomedical device trials. The program is supported by a Research Program Administrator, two pre-award Research Administrators, a Clinical Research Supervisor, and a staff of Clinical Research Assistants. The department maintains clinical research space within emergency departments as well as fully-equipped basic science laboratories, and has ongoing collaborations with Brown University and other hospital departments.The main focus areas of the Department of Emergency Medicine research program are (1) injury prevention and control, (2) cardiovascular, neurologic, and resuscitation science, (3) pediatric, age, and gender specific care, and (4) healthcare systems science.
Diagnostic Imaging Research Contact: Glenn A. Tung, MD	Research - Diagnostic Imaging Research Resources Clinical Trials Support, Diagnostic Imaging The Division of Imaging Research has successfully completed both federally-funded and industry-sponsored research trials. The Division has participated in cancer imaging trials that include screening studies and studies looking at the efficacy of various imaging modalities.The Division has participated in studies with pharmaceutical companies on phase I, II, and III trials. These studies include both diagnostic and cancer treatment drugs. We have also worked with various device manufacturers that are seeking pre-market and post-market Food and Drug Administration approval for medical devices. In addition, the Division has participated in several blood biomarker studies.
BioBank, BrainTissue Contact: Ivana Delalle, MD, PhD	BioBank - Brain Tissue Resource Center Clinical Research Resources, Brain Tissues A brain tissue resource center (BTRC) (often referred to as a brain bank) is a collection of human tissue specimens from the nervous system: the brain, spinal cord, muscle and peripheral nerves. The facility is engaged in a variety of functions; rapid specimen transport, tissue processing, state-of-the-art preservation methods and database management. A variety of experts, research doctors, neuropathologists and laboratory specialists, plan and manage the BTRC. Strict protocol is imposed on outside vendors whenever their services are required, particularly with respect to rapid specimen transport.
IDeA COBRE on Opioids and Overdose Contact: Heather Wunschel	IDeA COBRE on Opioids and Overdose Clinical Research Resources, Opioids and Overdose The COBRE aims to develop and sustain a vibrant interdisciplinary center of research excellence on opioids and overdose. The COBRE will support the selection, mentorship, and career development of junior investigators performing research in the area of opioids and overdose. The Center will bring together an interdisciplinary group of accomplished senior scientists, promising junior investigators, and distinguished advisors and collaborators to work closely together to establish a center of excellence in an area of clinical research that is of critical public health importance.

Facility	Instrument/Service/Resource Unit
Center for AIDS Research (CFAR) - Providence/Boston Contact: Vicki C. Godleski	Research - Basic Sciences, HIV research Clinical Research Service, HIV Research The Basic Sciences Core builds and supports collaborative research and synergy between basic, translational clinical and behavioral investigators as well as other multidisciplinary scientists, by providing laboratory, consultation and mentoring services. Services include; Viral load measurement, STI testing, Genital tract specific assays, Biologic specimens collection techniques, HIV-1 and 2 drug resistance assays, Identification of HIV dependency factors (HDF), Cell culture, Viral stocks, Immune activation, Laboratory Consulting, Training and Mentoring. Additional assays may be developed as necessary, please contact the Core Director.
Infectious Diseases Clinical Research Program - The Miriam Hospital Contact: Pamela Poethke, RN	Research - Infectious Diseases Clinical Research Resources Clinical Trials Support, HIV and HCV The Miriam Hospital clinical trials unit offers the newest treatments for HIV and hepatitis C (HCV) infections. The experienced research staff consists of physicians, nurses, a data manager, a regulatory assistant and laboratory personnel. The unit conducts trials offered through the AIDS Clinical Trials Group (ACTG) network, funded by the National Institutes of Health, in association with the Partners/Harvard AIDS Clinical Trials Unit. The priority of ACTG studies is to evaluate new strategies for treating HIV infection and preventing HIV complications, and to evaluate new treatments for hepatitis C. Other studies offered through our clinical trials unit include pharmaceutical studies to treat hepatitis C, prevent C. difficile infection, and test new methods of diagnosing HIV infection.
Center for AIDS Research (CFAR) - Providence/Boston Contact: Vicki C. Godleski	Research - BioBehavioral Research Support, HIV Clinical Research Service, HIV Research The BioBehavioral Sciences Core (BBSC) provides help with participant recruitment, enrollment, and retention to advance biobehavioral research; services to support innovative medical and behavioral data collection approaches, including methods, tools, and assessments; expertise and services related to regulatory and human subjects’ compliance, and the ethical conduct of research for vulnerable populations; provide expertise and services to support the development, implementation, and evaluation of interventions to improve HIV prevention and continuum of care outcomes; specialized services for the conduct of implementation science research and for performing cost-effectiveness research; PrEP related services and information; and guidance and support for accessing EMR data.
Center for AIDS Research (CFAR) - Providence/Boston Contact: Vicki C. Godleski	Administration - CFAr Clinical Research Service, CFAR Coordinating Center The CFAR Administrative Core has built an HIV infrastructure across the partner institutions and provides oversight of the entire CFAR community with responsibility for sound management, organizational structure, appropriate resource allocation, evaluation and the promotion of inter/multi-disciplinary translational HIV research. Through efficient communication to CFAR membership, the Core manages a highly interactive and collaborative scientific environment across geographically dispersed settings in domestic, national and international sites.
Center for AIDS Research (CFAR) - Providence/Boston Contact: Vicki C. Godleski	Research - Substance Use Research Core, HIV Clinical Research Service, HIV Research The Substance Use Research Core (SURC) offers services and expertise on the methods needed to conduct effective Substance Use (SU)/HIV research. The SURC offers expertise in innovative methods for assessing and analyzing SU, and developing and implementing interventions to address alcohol, tobacco, and other drug use. Services include: expertise in developing research projects that address gaps in SU/HIV priority issues both domestically and globally; advice on training on the most appropriate methods for assessing SU; advice and training on incorporating effective and up-to-date SU interventions; and guidance on addressing ethical issues with SU research including input on IRB protocols and human subject sections of grant applications and access to group trainings.
Center for AIDS Research (CFAR) - Providence/Boston Contact: Vicki C. Godleski	Research - Biostatistics, HIV Clinical Research Service, HIV Research The Biostatistics Core ensures that all research undertaken by Center for AIDS Research (CFAR) investigators uses optimal methods for study design and analysis. The Core provides guidance and collaboration in developing grant applications; collaboration on study design, conduct, and statistical analyses; statistical training and mentoring; facilitation of linkages to potential collaborators; provides guidance and support for accessing EMR data; facilitates access to HIV databases from other centers/programs, and develops novel statistical and epidemiological methods for HIV research.
IDeA COBRE for Stress, Trauma, and Resilience (STAR) Contact: Gayle Fraser	STAR COBRE for Stress, Trauma, and Resilience Clinical Research Resources, Stress, Trauma, and Resilience The STAR COBRE aims to foster transdisciplinary collaborations within Brown University, to provide training opportunities for researchers and clinicians who want to address stress and trauma, to enhance partnerships with local and community stakeholders, and to affect policy at the local, national, and international levels.
Sexually Transmitted Disease Clinic Contact:	Research - Infectious Diseases Clinical Research The Miriam Hospital clinical trials unit offers the newest treatments for HIV and hepatitis C (HCV) infections. The experienced research staff consists of physicians, nurses, a data manager, a regulatory assistant and laboratory personnel. We conduct trials offered through the AIDS Clinical Trials Group (ACTG) network, funded by the National Institutes of Health, in association with the Partners/Harvard AIDS Clinical Trials Unit. The priority of ACTG studies is to evaluate new strategies for treating HIV infection and preventing HIV complications, and to evaluate new treatments for hepatitis C.
IDeA COBRE for Stress, Trauma, and Resilience (STAR) Contact: Gayle Fraser	Community Collaborative Core Clinical Research Resources The Community Collaborative Core will support the overall goals of the STAR COBRE by building critical infrastructure to support clinically significant and highly innovative research with vulnerable children and adults with stress and trauma histories.
IDeA COBRE for Stress, Trauma, and Resilience (STAR) Contact: Gayle Fraser	Technology, Assessment, Data, and Analysis Core (TADA) Clinical Research Resources The TADA Core will offer unique expertise in leveraging technology for observational, laboratory, and intervention studies critical for (1) obtaining ecologically-valid data on stress exposures, behavioral and physiological responses with high temporal resolution, (2) novel approaches to digital phenotyping, and (3) electronically-delivered adaptive interventions.

Facility	Instrument/Service/Resource Unit
Clinical Pharmacokinetics Research Laboratory Contact: Fatemeh Akhlaghi	Pharmacokinetics, Bioanalytical support Clinical Research Resources, Pharmakokinetics The Clinical Pharmacokinetics Research Laboratory is equipped with two UPLC-MS/MS and one quadrupole-time-of-flight mass spectrometer (ESI-QTOFMS) systems capable of measuring the concentration of small molecule xenobiotics and their metabolites as well as endogenous or therapeutic peptides and proteins. To date, this laboratory has developed various assays for the quantification of up to 60 different compounds and validated these assays according to guidelines by the Food and Drug Administration of the United States. The laboratory is proficient in providing analytical support for on-going pre-clinical and clinical studies and during different stages of a drug development project.
Clinical Pharmacokinetics Research Laboratory Contact: Fatemeh Akhlaghi	Pharmacokinetics Consultation Clinical Research Resources, Pharmakokinetics The laboratory is capable of providing support for the design and implementation of pre-clinical and clinical pharmacokinetics studies as well as design and conduct of ancillary in vitro studies (protein binding and microsomal incubations to characterize phase 1 or 2 metabolism). With extensive background in pharmacokinetics modeling and simulation using NONMEM, WinNonlin and Simcyp softwares, the laboratory can provide a valuable resource for on-going or completed clinical studies. Additional data analysis capabilities includes in-vitro in-vivo correlation (IVIVC) and bioequivalence analysis.
Institute for Immunology and Informatics (iCubed) Contact: Barbara Muhitch	Research - Immunology and Informatics Resources Clinical Research Resources, Immunology and Informatics iCubed’s research focuses on creating new and safer vaccines. We also explore new methods of predicting and treating adverse immune responses. iCubed supports a wide variety of training efforts that provide opportunities to advance women and students in science which include unique technology toolkits and hands-on laboratory experiences.

Facility

Instrument/Service/Resource Unit

Clinical Pharmacokinetics Research Laboratory

Contact: Fatemeh Akhlaghi

Pharmacokinetics, Bioanalytical support

Clinical Research Resources, Pharmakokinetics
The Clinical Pharmacokinetics Research Laboratory is equipped with two UPLC-MS/MS and one quadrupole-time-of-flight mass spectrometer (ESI-QTOFMS) systems capable of measuring the concentration of small molecule xenobiotics and their metabolites as well as endogenous or therapeutic peptides and proteins. To date, this laboratory has developed various assays for the quantification of up to 60 different compounds and validated these assays according to guidelines by the Food and Drug Administration of the United States. The laboratory is proficient in providing analytical support for on-going pre-clinical and clinical studies and during different stages of a drug development project.

Clinical Pharmacokinetics Research Laboratory

Contact: Fatemeh Akhlaghi

Pharmacokinetics Consultation

Clinical Research Resources, Pharmakokinetics
The laboratory is capable of providing support for the design and implementation of pre-clinical and clinical pharmacokinetics studies as well as design and conduct of ancillary in vitro studies (protein binding and microsomal incubations to characterize phase 1 or 2 metabolism). With extensive background in pharmacokinetics modeling and simulation using NONMEM, WinNonlin and Simcyp softwares, the laboratory can provide a valuable resource for on-going or completed clinical studies. Additional data analysis capabilities includes in-vitro in-vivo correlation (IVIVC) and bioequivalence analysis.

Institute for Immunology and Informatics (iCubed)

Contact: Barbara Muhitch

Research - Immunology and Informatics Resources

Clinical Research Resources, Immunology and Informatics
iCubed’s research focuses on creating new and safer vaccines. We also explore new methods of predicting and treating adverse immune responses. iCubed supports a wide variety of training efforts that provide opportunities to advance women and students in science which include unique technology toolkits and hands-on laboratory experiences.

Facility	Instrument/Service/Resource Unit
Clinical Research and Development Service Contact: Gaurav Choudhary	Research - Clinical Research and Development Service Resources Clinical Research Resources, Veteran Population The VA Research's fundamental mission is to advance the healthcare of Veterans. The Providence VA Medical Center has a large funded research and development program, including studies in Rehabilitation Medicine, Cardiology, Mental Health, Neuroscience, Substance Abuse, Health Services & Economics, and Pulmonary Disease. These studies enhance our ability to provide state-of-the-art techniques and treatments to our patients. At the same time, the research program helps Providence VAMC recruit and retain the highest quality health care professionals who treat our Veterans in the VA Clinics on a daily basis.

Facility

Instrument/Service/Resource Unit

Clinical Research and Development Service

Contact: Gaurav Choudhary

Research - Clinical Research and Development Service Resources

Clinical Research Resources, Veteran Population
The VA Research's fundamental mission is to advance the healthcare of Veterans. The Providence VA Medical Center has a large funded research and development program, including studies in Rehabilitation Medicine, Cardiology, Mental Health, Neuroscience, Substance Abuse, Health Services & Economics, and Pulmonary Disease. These studies enhance our ability to provide state-of-the-art techniques and treatments to our patients. At the same time, the research program helps Providence VAMC recruit and retain the highest quality health care professionals who treat our Veterans in the VA Clinics on a daily basis.

DNA Sequencing

Facility	Instrument/Service/Resource Unit
Genomics Facility - Brown Contact: Christoph Schorl, PhD	DNA Sequencer Illumina - NextSeq550 The NextSeq550 Next Generation Sequencing system is a high throughput bench top sequencer that provides accurate fast sequencing data, and by employing flexible run configurations it can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth. Reagents are available in a mid-output and a high output mode resulting in maximum cluster numbers of 400 million (high output) and 130 million (mid output) clusters (reads).

Facility

Instrument/Service/Resource Unit

Genomics Facility - Brown

Contact: Christoph Schorl, PhD

DNA Sequencer

Illumina - NextSeq550
The NextSeq550 Next Generation Sequencing system is a high throughput bench top sequencer that provides accurate fast sequencing data, and by employing flexible run configurations it can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth. Reagents are available in a mid-output and a high output mode resulting in maximum cluster numbers of 400 million (high output) and 130 million (mid output) clusters (reads).

Facility	Instrument/Service/Resource Unit
Biorepository Core Contact: Evgeny Yakirevich	DNA Sequencer Illumina iseq 100 Illumina assay instrument FS10001514 Genotypic profiling by whole genome sequencing 16S metagenomic sequencing. Antimicrobial resistance detection by gene sequencing

Facility

Instrument/Service/Resource Unit

Biorepository Core

Contact: Evgeny Yakirevich

DNA Sequencer

Illumina iseq 100 Illumina assay instrument FS10001514
Genotypic profiling by whole genome sequencing 16S metagenomic sequencing. Antimicrobial resistance detection by gene sequencing

Facility	Instrument/Service/Resource Unit
PC - Hickey Hall Labs Contact: Charles Toth	DNA Sequencer ABI Prism 3100 Genetic Analyzer Used for DNA sequencing and fragment analysis. The ABI PRISM 3100 Genetic Analyzer is an automated capillary electrophoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled DNA fragments in one run.

Facility

Instrument/Service/Resource Unit

PC - Hickey Hall Labs

Contact: Charles Toth

DNA Sequencer

ABI Prism 3100 Genetic Analyzer
Used for DNA sequencing and fragment analysis. The ABI PRISM 3100 Genetic Analyzer is an automated capillary electrophoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled DNA fragments in one run.

Facility	Instrument/Service/Resource Unit
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	DNA Sequencer Ion Gene Studio/S5 with Ion Chef/ThermoFisher Next Generation Sequencer
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	DNA Sequencer Qiagen Pyromark Q96 ID Quantitative analysis of genetic/epigenetic DNA modifications using Pyrosequencing technology

Facility

Instrument/Service/Resource Unit

Genomics Laboratory - Providence VA Medical Center

Contact: John McGeary, PhD

DNA Sequencer

Ion Gene Studio/S5 with Ion Chef/ThermoFisher
Next Generation Sequencer

Genomics Laboratory - Providence VA Medical Center

Contact: John McGeary, PhD

DNA Sequencer

Qiagen Pyromark Q96 ID
Quantitative analysis of genetic/epigenetic DNA modifications using Pyrosequencing technology

Engineering

Facility	Instrument/Service/Resource Unit
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Chemical Hood Wet chemistry workbenches equipped with fume hoods for lithography The Microelectronics Facility has a number of wet chemistry workbenches equipped with fume hoods for chemical processing (cleaning, wet etching, electroplating, anodization, etc) employed in the fabrication of semiconductor and nanomaterials devices. These hoods also house spinners for applying photoresist for lithography and other polymers.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Lithography - Mask Aligner Karl Suss MJB3 UV300 Mask Aligner for Lithography The Karl Suss MJB3 UV300 3-inch mask aligner is designed for high-resolution photolithography, with a 350 W mercury lamp and Suss diffraction-reducing exposure optics. The primary exposure wavelengths of 365 or 403 nm lead to roughly 1 µm minimum feature size. For smaller feature sizes, electron-beam lithography is available in the Electron Microscope Facility.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Lithography - Maskless Aligner Heidleberg MLA 150 Maskless Aligner MLA150 Maskless Aligner is a high-speed, direct-write, optical lithography system to fabricate nano- and micro-patterned materials and surfaces at low-cost and over large areas. The instrument comprises two laser illumination modules, optical systems to steer the laser beams and control exposure, integrated cameras for high-accuracy alignment to pre-existing structures, automated translation stage, real-time pneumatic autofocus, and a class 10 climate controlled chamber to achieve photo-lithographically defined structures with feature size ≤1μm.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Lithography - Flexible Mylar-Mask Newport-Oriel flexible Mylar-Mask Lithography System Newport-Oriel flexible Mylar-Mask Lithography System is designed for printing from flexible Mylar masks onto substrates as large as 4” x 4” square or 5” diameter. It is capable of printing 5-micron features with placement and alignment to approximately 2 microns with appropriate alignment marks.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Evaporator - Thin Film Deposition Electron Beam Evaporator This system deposits thin films of inorganic materials, usually metals, from a four-pocket electron-beam source inside a high vacuum (cryopumped) chamber. Deposition rates of up to 0.1 micron/minute are possible for some materials. This system can easily accomodate relatively large, 3-D objects and has substrate heating to 300 C for flat substrates. Restrictions on what materials may be evaporated are somewhat less stringent than for our other evaporators.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Lithography - Imprinter NIL Technology Nano Imprinter The Nano Imprinter system offers thermal and UV nanoimprint and imprinting in vacuum if needed. The system transfers the patterns, usually nanohole or nanopillar arrays, from a silicon stamp to a nanoimprint-polymer-coated substrate by applying heat and pressure.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Evaporator - Thin Film Deposition Angstrom Organic Evaporator The Angstrom Resitive-heated Evaporator is a thermal evaporator coupled with a nitrogen glove box. It is for depositing thin organic and inorganic films when either the deposition material or the substrate is adversely affected by moisture or oxygen. Samples and/or evaporants are loaded from a nitrogen glove box maintained at under 10 ppm of oxygen and under 1 ppm of water vapor.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Deposition System - Thin Film Deposition Cambridge NanoTech Atomic Layer Deposition System The Cambridge NanoTech Fiji F200 series Atomic Layer Deposition System is a modular medium to high-vacuum ALD system that accommodates a wide range of deposition modes. This tool is currently configured with precursors for metallic and oxide layers of Hafnium, Aluminum, and Tungsten.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Deposition System - Thin Film Deposition Plasmatherm Model 790, Fluorine Chemistry Reactive Ion Etching Plasma Enhanced Chemical Vapor Deposition System (RIE PECVD) The PlasmaTherm Model 790 RIE-PECVD system has a computer-controlled single-wafer turbopumped chamber that provides reactive ion etching (RIE) and plasma-enhanced chemical vapor deposition (PECVD) capabilities. RIE is accomplished using fluorine-chemistry gases (CF4, CBrF3, CHF3, SF6, etc.) with up to 500 W RF power. PECVD capability provides low-temperature (up to 350 C) SiO2 and Si3N4 deposition from silane chemistry.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Profilometer - Characterization DekTak3 Profilometer The DekTak3 is a computer-controlled surface profile measuring system, which accurately measures step heights from below 50 A to over 50 µm by moving a diamond-tipped stylus over the surface. Equipped with video camera and surface profile analysis software.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Analytical Prober Karl Suss 200mm Precision Manual Analytical Prober 200mm Precision Manual Analytical Prober. The PM8 provides the stability and resolution required for precise probe positioning. Manual submicron semiconductor failure analysis and in-process testing probe station. Submicron precision and stability with a fine-glide chuck stage and 8 in. x 8 in. X-Y movement. As fast as a motorized stage but with the simplicity and resolution of a manual system. Vacuum Chuck: 8 in. dia. Mitutoyo high magnification microscope with two long working distance objectives. Microscope lifts for easier access to the probes and device under test. The station is fitted with six Wentworth micromanipulators having tungsten needle probes with 3 micron tips.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Deposition System - Thin Film Deposition Lesker Lab 18 thin film deposition system The Lab-18 thin film deposition system is a combined electron-beam evaporator and RF sputtering Physical Vapor Deposition (PVD) system that makes high quality, research-grade metallic, semiconducting, and dielectric thin films. The Lab 18 includes a four-pocket electron beam evaporator as well as two 2” magnetron sputter sources. Two mass flow regulated gas lines provide for control of chamber pressure and gas composition for reactive sputtering.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Plasma Etching System Inductively Coupled Plasma RIE System The SPTS LPX is an inductively-coupled plasma (ICP) reactive-ion etcher (RIE). It can etch high-aspect ratio structures in a wide range of materials, including silicon, silicon dioxide, and silicon nitride. This system is configured with a range of gases for reactive- and physical-ion etching, including SF6, C4F8, CF4, O2, and Ar. The LPX-ICP system is equipped with a single-wafer load-lock transfer arm for 100mm wafers; smaller wafers and small pieces may be processed on the system using a carrier wafer.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Characterization - Thin Film Deposition JA Woollam, M-2000 Ellipsometer Variable Angle Research Grade The JA Woollam, M-2000 Variable Angle Research Grade ellipsometer measures angle- and wavelength-dependent reflectivity and transmissivity data over a wide spectral range (from ultraviolet to near-infrared) that is analyzed to directly to determine optical properties of single- and multi-layer films (e.g., their thickness and complex wavelength-dependent refractive index), which provide insight into their underlying chemical, electronic, and mechanical properties.
Nanofabrication Central Facility (NCF) Contact: Michael Jibitsky	Furnace Wet and Dry Oxididation Quartz tube furnace for thermal oxide growth on silicon by a chemical reaction between the silicon and either dry oxygen or water vapor at atmospheric pressure. The typical temperature range for the oxidation of silicon for wafer fabrication 750˚C to 1100˚C. The tubes accommodate multiple 2 inch wafers.
NanoTools Facility Contact: Hector Garces	X-Ray Diffraction System, 2 Dimensional Bruker, D8, XPS This Bruker D8 Discover X-ray Diffraction System can be configured with a point and line source x-rays. A Vantec 500 2D area detector or a LinxEye (0D, 1D)detector can be used for the analysis. The system is suitable for texture, stress, and micro-x-ray diffraction as well as for orientation and epitaxy studies. The instrument has in-situ capabilities for studies at low/high temperature and under vacuum to study properties as a function of time, temperature, gas environment or vacuum. A capillary spinner stage is also available for investigations of air sensible materials with the sample prepared in a capillary glass.
NanoTools Facility Contact: Hector Garces	X-Ray Diffration System, High-Resolution Bruker, D8, XPS This Bruker D8 Discover X-ray Diffraction System includes a line source configuration system with a LinxEye (0D, 1D) detector and a Scintillator detector. The system is suitable to determine thickness, crystallographic structure and strain in epitaxially thin films as well as high resolution analysis of bulk materials. X-ray reflectometry (XRR), high resolution XRD, grazing incidence diffraction (GI-XRD), in-plane GID (IGID) and reciprocal space mapping (RSM) are some techniques that can be performed with this configuration. High resolution is obtained using parallel beam optics: Goebel mirror, monochromators, soller slits and analyzer crystals.
NanoTools Facility Contact: Hector Garces	Microscope - Atomic Force Asylum, MFP-3D, AFM The Asylum MFP-3D Origin atomic force microscope (AFM) is a multi-mode instrument. It is equipped with a closed loop operation in all three axes with high precision, resolution and low noise operation. The closed loop Z scanner eliminates hysteresis. The AFM provides a 90 µm X and Y range (< 0.5nm noise) and 15 µm Z range (Z sensor, 0.25 nm noise) and detector noise <15 pm. It also includes Inverted optical lever design that eliminates interference artifacts and a low-coherence infrared (860 nm) super-luminescent diode (SLD) light source (class 1M). Applications include dual AC resonance tracking (DART), electric force microscopy (EFM), force mapping mode, lateral force microscopy (LFM), magnetic force microscopy (MFM), kelvin probe force microscopy (KPFM), piezo-response force microscopy (PFM), and conductive AFM (C-AFM) with ORCA.
NanoTools Facility Contact: Hector Garces	Microscope - Confocal Raman Witec, Alpha 300 M+, CRM The Witec Alpha 300 Confocal Raman Microscope is equipt with different scan modes for local Raman spectra, mapping distribution of phases within a sample and with 3D image generation and depth profile of samples. The depth profiling and 3D imaging capabilities of the Alpha 300 provide the ability to analyze the interior of transparent samples without microtome sectioning or freeze etching. Multi-wavelength coupler for selection of 532 nm or 633 nm wavelength laser sources and a Ray Shield coupler for measurements of Raman spectra at low wave numbers. Fast Raman imaging with integration time in the milliseconds order per spectrum. A heating stage for in-situ measurements in the temperature range 25°C to 1400°C with a thermal stability of ± 0.3°C.
NanoTools Facility Contact: Hector Garces	Spectrometer - Electron Paramagnetic Resonance (EPR) Bruker, EMX+, EPR The Bruker EMX Electron Paramagnetic Resonance (EPR) Spectrometer is equipt for the Technique for the direct detection of paramagnetic species. The EMX EPR has a digital resolution of 24 bit center field, a field sweep up to 256,000 points, high-sensitivity resonator with irradiation window, dual channel, simultaneous detection of 1st and 2nd harmonic, and sensitivity S/N up to 2000:1. The system has a helium temperature control system for low temperature measurements in the temperature range 3.8 K (~-269 °C) to 300 K (~27°C). Applications span a wide range of areas an include kinetics of radical reactions, oxidation and reduction process, polymerization reactions, degradation of compounds by light, organometallic compounds and catalysis, free radicals, and antioxidants and radical scavengers.
Joint Engineering Physics Instrument Shop (JEPIS) Contact: Michael Packer	Fabrication - Machine Shop Service that provides machine shop capabilities The Joint Engineering Physics Instrument Shop (JEPIS) is a specialized service center providing assistance in the development of experimental instruments, research projects and instructional classes. The JEPIS services are offered to faculty, students and staff on the Brown University campus, and is also available to outside academic/hospital users. JEPIS offers all of the basic machine shop capabilities, including Wire EDM and Rapid Prototype printing. JEPIS also offers CNC design consultation, hands on training and instruction through non-credit basic machine shop course and an advanced course geared toward Graduate Students. The Student Shop provides students with a strong base for their experimental studies. The JEPIS staff is available to give general advice and assistance to the Brown Students and Staff in the finding of sources for the materials and devices they require for their projects or instructional classes. Access to the facility is on a user fee basis of an hourly fee for labor plus materials.

Facility	Instrument/Service/Resource Unit
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Atomic Force Shimadzu SPM-9700 Scanning probe microscope for atomic force microscopy, magnetic force microscopy, liquid cell analysis, Kelvin force microscopy, I/V curves, nanomechanical measurements
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Spectrometer - Energy Dispersive X-Ray Fluorescence Shimadzu EDX-8100 Energy dispersive X-ray fluorescence of powders, solids and liquids. Ambient pressure, vacuum and He atmosphere
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Scanning Transmission Electron JEOL F200 Scanning transmission electron microscope with cryogenic capabilities, direct electron camera (Gatan K3), Energy dispersive X-ray spectroscopy (JEOL), electron energy loss spectroscopy (Gatan). Sub nanometer resolution, 80 and 200 kV operation, TEM, STEM and diffraction
MakerspaceURI Contact: makerspace@etal.uri.edu	Tools - Router - computer numerical control (CNC) Inventables X-Carve
MakerspaceURI Contact: makerspace@etal.uri.edu	Computers DELL high-performance PCs (4), Alienware PC (3)
MakerspaceURI Contact: makerspace@etal.uri.edu	Cutter - Laser GCC Laserpro Spirit LS
MakerspaceURI Contact: makerspace@etal.uri.edu	Sewing Machines/ Embroidery Brother XM2701 Sewing Machine (2), Brother PE-770 Embroidery System

Flow Cytometry

Facility	Instrument/Service/Resource Unit
Flow Cytometry and Sorting Facility Contact: Kevin Carlson	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences FACS Aria III Cell counting, sorting, biomarker detection. The FACSAria has five air-cooled lasers at 351nm, 488nm, 561nm, 633nm, and 407nm allowing multicolor analysis of up to 20 parameters. It can sort 1 to 4 populations simultaneously and is equipped with an Automated Cell Deposition Unit, an Aerosol Management System and a refrigeration system. It can accept various sample input tubes including 1 mL microtubes, 12 x 75-mm test tubes, and 15 mL tubes into the sample and collection chambers.
Flow Cytometry and Sorting Facility Contact: Kevin Carlson	Flow Cytometer - Benchtop Analyzer Cytek Aurora The Cytek Aurora can analyze similar and very similar spectral emitting fluorochromes. Instead of analyzing small chunks of fluorescence and calculating fluorescence spill over, the Cytek Aurora looks at the full spectrum emitence of each fluorochrome. This allows colors that would normally be unusable with each other to be run in tandem. The Cytek Aurora can analyze up to 38 fluorochromes, 41 parameters (FSC, 488 SSC, 405 SSC).
Flow Cytometry and Sorting Facility Contact: Kevin Carlson	Fluorescence Acticated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences FACS Celesta Cell counting, biomarker detection. The FACSCelesta has three air-cooled lasers at 488nm, 561nm and 407nm allowing multicolor analysis of up to 15 parameters that has an attached high throughput system (HTS) that allows users to analyze samples directly from a 96 well plate.

Facility

Instrument/Service/Resource Unit

Flow Cytometry and Sorting Facility

Contact: Kevin Carlson

Fluorescence Activated Cell Sorter (FACS)

Becton Dickinson (BD) Biosciences FACS Aria III
Cell counting, sorting, biomarker detection. The FACSAria has five air-cooled lasers at 351nm, 488nm, 561nm, 633nm, and 407nm allowing multicolor analysis of up to 20 parameters. It can sort 1 to 4 populations simultaneously and is equipped with an Automated Cell Deposition Unit, an Aerosol Management System and a refrigeration system. It can accept various sample input tubes including 1 mL microtubes, 12 x 75-mm test tubes, and 15 mL tubes into the sample and collection chambers.

Flow Cytometry and Sorting Facility

Contact: Kevin Carlson

Flow Cytometer - Benchtop Analyzer

Cytek Aurora
The Cytek Aurora can analyze similar and very similar spectral emitting fluorochromes. Instead of analyzing small chunks of fluorescence and calculating fluorescence spill over, the Cytek Aurora looks at the full spectrum emitence of each fluorochrome. This allows colors that would normally be unusable with each other to be run in tandem. The Cytek Aurora can analyze up to 38 fluorochromes, 41 parameters (FSC, 488 SSC, 405 SSC).

Flow Cytometry and Sorting Facility

Contact: Kevin Carlson

Fluorescence Acticated Cell Sorter (FACS)

Becton Dickinson (BD) Biosciences FACS Celesta
Cell counting, biomarker detection. The FACSCelesta has three air-cooled lasers at 488nm, 561nm and 407nm allowing multicolor analysis of up to 15 parameters that has an attached high throughput system (HTS) that allows users to analyze samples directly from a 96 well plate.

Care New England - Women and Infants Hospital

Facility	Instrument/Service/Resource Unit
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences FACSCanto Analytical Flow Cytometer: 2 lasers, 6 colors
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Fluorescence Activated Cell Sorter (FACS) Thermo Scientific Attune NXT Analytical flow cytometer: 4 lasers, 14 colors, with 96 well plate handler capability

Facility

Instrument/Service/Resource Unit

Molecular Biology and Imaging Core

Contact: Sunil Shaw, PhD

Fluorescence Activated Cell Sorter (FACS)

Becton Dickinson (BD) Biosciences FACSCanto
Analytical Flow Cytometer: 2 lasers, 6 colors

Molecular Biology and Imaging Core

Contact: Sunil Shaw, PhD

Fluorescence Activated Cell Sorter (FACS)

Thermo Scientific Attune NXT
Analytical flow cytometer: 4 lasers, 14 colors, with 96 well plate handler capability

CharterCARE - Roger Williams Medical Center

Facility	Instrument/Service/Resource Unit
Roger Williams Medical Center Resources Contact: John W. Morgan, Ph.D.	Flow Cytometry Core Flow Cytometry and Cell Sorting The Flow Cytometry Core facility is dedicated to advancing research projects requiring cell sorting and/or analysis of cell populations by flow cytometry. The core offers training on and access to shared use instrumentation for analytical flow cytometry, services for fluorescence activated cell sorting (FACS) as well as technical consultation and support on experimental design, execution, and data analysis.

Facility

Instrument/Service/Resource Unit

Contact: John W. Morgan, Ph.D.

Flow Cytometry Core

Flow Cytometry and Cell Sorting
The Flow Cytometry Core facility is dedicated to advancing research projects requiring cell sorting and/or analysis of cell populations by flow cytometry. The core offers training on and access to shared use instrumentation for analytical flow cytometry, services for fluorescence activated cell sorting (FACS) as well as technical consultation and support on experimental design, execution, and data analysis.

Facility	Instrument/Service/Resource Unit
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson - Accuri Flow Cytometer This system is equipped with a blue and red laser, two light scatter detectors, and fluorescence detectors with optical filters optimized for FITC, PE, PerCP, and APC. The user does not need to adjust detector voltages and there is a wide dynamic range of digitally collected data (7.2 decades).
Flow Cytometry Core - Rhode Island Hospital Contact: Mark Dooner	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences LSRII Analyzer/Flow Cytometer Digital, 4 laser (355nm, 405nm, 488nm and 635nm) flow cytometric analysis system with 10 fluorescent channels plus 2 scatter channels. BD FACSDiva software with digital compensation and ModFit software for cell cycle analysis.
Flow Cytometry Core - Rhode Island Hospital Contact: Mark Dooner	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences Influx Cell Sorter 5 laser system (355nm, 405nm, 488nm, 561nm and 638nm) for simultaneous detection of up to 14 parameters. Sorts up to 4 different cell populations. Capable of single cell deposition into many different sized culture plates (96 or 384 well plates, etc). Equipped with a small particle objective for FSC size determination down to 0.5 micrometers. Enclosed in a biosafety hood. The sorter separates particles on the detection of fluorescent markers on particles such as cells, chromosomes, bacteria or nuclei. Then the collected cells are suitable for downstream analysis either in molecular applications like protein assays and PCR, or used in viable assays such as tissue culture or injected into in vivo models. Generated data may also be used to determine percentages of interrogated populations. The sorter can separate populations into tubes (1.5ml, 4ml, 15ml, 50ml), multi-well plates (6, 24, 48, 96, 384), or directly onto microscope slides.
Flow Cytometry Core - Rhode Island Hospital Contact: Mark Dooner	Mass Cytometry Fluidigm Helios (CyTOF) Mass Cytometry Mass cytometry combines the technologies of flow cytometry with that of mass spectrometry. It applies nebulization, aerosolization and ionization to cells labeled with heavy metal isotope-conjugates, allowing for ion separation based on their mass:charge ratio and quantification of each isotope on a per cell basis ultimately determining properties of a cell population at a single-cell resolution. The technique of mass cytometry is powerful, as it can analyze more cellular targets without the problem of compensation as seen in flow cytometry. The Helios is capable of simultaneously analyzing up to more than 50 parameters both on cell surface and intracellularly.
Flow Cytometry Core - Rhode Island Hospital Contact: Mark Dooner	Training and Technical Assistance Service

Facility	Instrument/Service/Resource Unit
Animal Models Core Contact: Gerard Nau, MD	Flow Cytometer Amnis CellStream Luminex Flow Cytometer The CellStream flow cytometer has single tube and 96 well plate capabilities with automatic compensation. Equipped with 405nm, 488nm, 532nm, and 642nm lasers and 16 fluorescent channels. Handles BSL-1 and fixed samples from BSL-2 experiments.

Facility

Instrument/Service/Resource Unit

Animal Models Core

Contact: Gerard Nau, MD

Flow Cytometer

Amnis CellStream Luminex Flow Cytometer
The CellStream flow cytometer has single tube and 96 well plate capabilities with automatic compensation. Equipped with 405nm, 488nm, 532nm, and 642nm lasers and 16 fluorescent channels. Handles BSL-1 and fixed samples from BSL-2 experiments.

Facility	Instrument/Service/Resource Unit
PC - Hickey Hall Labs Contact: Charles Toth	Fluorescence Activated Cell Sorter (FACS) Flow Cytometer- Accuri C6 The BD Accuri C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable.

Facility

Instrument/Service/Resource Unit

PC - Hickey Hall Labs

Contact: Charles Toth

Fluorescence Activated Cell Sorter (FACS)

Flow Cytometer- Accuri C6
The BD Accuri C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable.

Facility	Instrument/Service/Resource Unit
RWU Lab Spaces Contact: Nancy Breen	Fluorescence Activated Cell Sorter (FACS) Millipore Guava easyCyte Flow Cytometer Cell sorting and analysis

Facility

Instrument/Service/Resource Unit

Salve Regina Biology and Biomedical Sciences

Contact: Nancy Breen

Fluorescence Activated Cell Sorter (FACS)

Millipore Guava easyCyte Flow Cytometer
Cell sorting and analysis

Facility	Instrument/Service/Resource Unit
Salve Regina Biology and Biomedical Sciences Contact: Anne Reid	Fluorescence Activated Cell Sorter (FACS) Millipore Guava EasyCyte 8 Flow Cytometer Single blue (488 nm), dual blue and red (642 nm), or triple blue, red, and violet (405 nm) excitation lasers provide up to 12 simultaneous detection parameters, including 10 fluorescent colors plus forward and side scatter for size and granularity determination. The guava easyCyte family also meets your sample throughput needs by offering both single sample and multi-sample processing.

Facility

Instrument/Service/Resource Unit

Contact: Anne Reid

Fluorescence Activated Cell Sorter (FACS)

Millipore Guava EasyCyte 8 Flow Cytometer
Single blue (488 nm), dual blue and red (642 nm), or triple blue, red, and violet (405 nm) excitation lasers provide up to 12 simultaneous detection parameters, including 10 fluorescent colors plus forward and side scatter for size and granularity determination. The guava easyCyte family also meets your sample throughput needs by offering both single sample and multi-sample processing.

Facility	Instrument/Service/Resource Unit
Marine Science Research Facility Contact: Dawn Outram	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences Influx Flow Cytometer and Cell Sorter Measures light scatter and fluorescence of cells. Sorts cells based on measured properties.
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Fluorescence Activated Cell Sorter (FACS) Becton Dickinson (BD) Biosciences FACS Verse Flow cytometer Fluorescence-activated cell sorting
Cell Analysis and Sorting Core Contact: Alan Rothman, M.D., Research Professor	Flow Cytometer Miltenyi MACSQuant-10 Analytical Flow Cytometer Analytical flow cytometry- 3 lasers, 10 parameters (8 colors, FSC, SSC)
Cell Analysis and Sorting Core Contact: Alan Rothman, M.D., Research Professor	Cell Sorter - Magnetic-Activated Cell Sorter (MACS) Miltenyi AutoMACS Automated cell sorter for cell suspensions labeled with magnetic beads.
Cell Analysis and Sorting Core Contact: Alan Rothman, M.D., Research Professor	Flow Cytometer BD LSRII Flow Cytometer Analytical flow cytometric analysis; 4 lasers (blue (2), violet (4), red (3) and yellow green (5)) which allows for analysis of up to 14 different fluors (# listed in parentheses for each laser)

Facility	Instrument/Service/Resource Unit
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Flow Cytometer - Magnetic-Activated Cell Sorter (MACS) Miltenyi MACSQuant Analyzer 7 Flow Cytometer The MACSQuant Analyzer is a powerful benchtop flow cytometer for highly sensitive, multiparameter cell analysis. MACSQuant Analyzers offer fully automated instrument setup via pre-set calibration and compensation programs. Automated startup, shutdown, and cleaning cycles make for hassle-free instrument housekeeping. The MACSQuantify Software provides a powerful and easy-to-use user interface for instrument control, data acquisition, and fully automated analysis.

Facility

Instrument/Service/Resource Unit

IDeA COBRE for CardioPulmonary Vascular Biology Core

Contact: Valeria Zarate

Flow Cytometer - Magnetic-Activated Cell Sorter (MACS)

Miltenyi MACSQuant Analyzer 7 Flow Cytometer
The MACSQuant Analyzer is a powerful benchtop flow cytometer for highly sensitive, multiparameter cell analysis. MACSQuant Analyzers offer fully automated instrument setup via pre-set calibration and compensation programs. Automated startup, shutdown, and cleaning cycles make for hassle-free instrument housekeeping. The MACSQuantify Software provides a powerful and easy-to-use user interface for instrument control, data acquisition, and fully automated analysis.

GeoChemistry

Facility	Instrument/Service/Resource Unit
Mass Spectrometry Facility (Brown Chemistry) Contact: Lisa Chen	Gas Chromatograph - Mass Spectrometer (GC - MS) Hewlett-Packard (Agilent) 6890 GC/5973 MSD This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass range 450 Da).
Mass Spectrometry Facility (Brown Chemistry) Contact: Lisa Chen	Liquid Chromatography - Mass Spectrometer (LC - MS) Agilent 6530 Mass QTOF This system consist of Agilent 1260 infinite HPLC and Agilent 1260 infinite nanoglow LC/chipcube nanoelectrospray MS interface, and 6530 Accurate Mass Q-TOF mass spectrometer. This instrument can detect and identify compounds in amounts smaller than two trillionths of a gram (2 picograms), making the Agilent 6530 a powerful tool for food safety, toxicology and other measurements where minute amounts of compounds must be identified.
Mass Spectrometry Facility (Brown Chemistry) Contact: Lisa Chen	Mass Spectrometer Thermo LXQ linear ion trap with Surveyor HPLC system The LXQ has capabilities similar to the conventional 3D ion trap such as LCQ Deca. It is based on linear ion trap technology that has faster cycle time. It is capable of data-dependent LC/MSn analysis.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Analyzer - Discrete Analyzer Westco Smartchem 200 Used to determine Nitrate/Nitrite, Phosphate, Ammonia, Silicate, and other nutrient concentrations in water samples and soil extracts.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Analyzer - Elemental Analyzer Thermo Scientific, FlashSmart EA Analysis of carbon, nitrogen, and sulfur concentration of solid samples
Environmental Chemistry Facility Contact: Marcelo Alexandre	Analyzer - Inorganic Carbon Analyzer UIC, INC Inorganic Carbon Analyzer Measures total inorganic carbon by automated acidification and coulometric detection in a wide variety of sample matrices. Solid samples ranging from low ppm to 100% inorganic carbon can be analyzed with this system.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Analyzer - Mercury Analyzer Milestone DMA-80 Direct Mercury Analyzer Determines mercury content of solid and liquid samples.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Analyzer - Water Isotope Picarro L1102-I Measures delta deuterium/hydrogen (D/H) ratio and delta 18O/16O in liquid water
Environmental Chemistry Facility Contact: Marcelo Alexandre	Sample Preparation - Freeze Dry System Labconco FreeZone 6 Liter For removing water and other solvents from frozen samples by sublimation.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Liquid Chromatography System Dionex DX500 Ion chromatography analysis of anions and cations in marine and freshwater samples
Environmental Chemistry Facility Contact: Marcelo Alexandre	Sample Preparation - Microwave Digestion System Milestone Ultrawave Used to digest solid samples (soils, sediments, rocks, plants, tissues), for elemental analysis.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Spectrometer - Atomic Absorption Spectrometer-Graphite Furnace (GFAAS) Perkin Elmer AAnalyst 600 Graphite Furnace This spectrometer is useful for measuring trace levels of metals in water and soil.
Environmental Chemistry Facility Contact: Marcelo Alexandre	Spectrometer - Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) Thermo Scientific iCAP 7400 DUO ICP-OES Analysis of most elements with atomic weights above 10.
Environmental Chemistry Facility Contact: Marcelo Alexandre	X-Ray Fluorescence - Handheld Innov-X Systems Alpha 4000 Handheld Non-destructive elemental analysis on a variety sample types for concentrations of some metals ranging from about 20 ppm to 10%.
Environmental Chemistry Facility Contact: Marcelo Alexandre	X-Ray Fluorescence - Handheld Olympus Delta Professional Handheld Non-destructive elemental analysis on a variety sample types for concentrations of elements greater than 10 ppm.
Mass Spectrometer Analytical Facility Contact: Soumen Mallick	Analyzer - Laser Ablation Photon-machines Analyte G2, 193 nm excimer laser ablation This 193 nm wave-length excimer laser allows us to perform in-situ analysis on a solid sample with a beam diameter as small as 3µm. To determine the elemental abundances the laser is coupled with the single collector inductively coupled plasma mass spectrometer ICPMS and to determine the isotope ratios the laser is coupled with multi collector ICPMS.
Mass Spectrometer Analytical Facility Contact: Soumen Mallick	Mass Spectrometer Thermo Scientific Quadrupole inductively coupled plasma mass spectrometer, ICP-MS, X-series-2 This is a single collector ICP-MS that allows us to measure precise elemental composition at sub-ppb level concentration.
Mass Spectrometer Analytical Facility Contact: Soumen Mallick	Mass Spectrometer Thermo Scientific Multi-collector inductively coupled plasma mass spectrometer, ICP-MS, Neptune Plus This is a multi-collector ICP-MS equipped with nine Faraday cups and eight ion counters, which allows us to monitor multiple masses simultaneously providing precise isotope ratio measurement.
Benedum Stable Isotope Facility Contact: Steven Clemens	Mass Spectrometer, Isotope Ratio ThermoFisher (Finnigan) 253+ dual inlet Isotope Ratio Mass Spectrometer with automated sample reaction device for up to 48 samples and with Kiel IV Carbonate interface and PoraPak purification trap. Instrument primarily dedicated to the measurement of the stable isotopes of carbon and oxygen on inorganic carbonates as well as clumped oxygen isotopes. The facility accommodates projects both internal and external to Brown including analysis of terrestrial and marine material. The vast majority of samples analyzed are funded through external grants but we also facilitate a significant number of small unfunded, exploratory, analyses to help construct proof-of-concept data sets in preparation for proposals.
Mass Spectrometer Analytical Facility Contact: Soumen Mallick	Workstation - Clean lab, metal free Pico Trace Clean Lab This Clean Lab is equipped with Millipore water filtration system that provides greater than 18MΩ Milli-Q water. The working stations in this Clean Lab meet ISO class 4 standard (equivalent to FS class 10) allowing us to perform ultra-low blank chemical analysis.
Electron Microprobe Facility Contact: Joseph S Boesenberg	Analyzer - Electron Microprobe Cameca SX100 Electron Microprobe Analyzer The Cameca SX-100 electron microprobe is equipped with 5 wavelength dispersive spectrometers (WDS) and a Rontec energy dispersive spectrometer (EDS).The WDS spectrometers each contain two or four diffracting crystals and presently include LTAP, TAP (2), LPET (2), PET, LLIF (2), LIF, LPCO, PC1 and PC2. The last three crystals are used to measure the light elements (boron to sodium). The absolute detection limits for the instrument are approximately 50-200 ppm, depending on the element. The EDS spectrometer is capable of detecting all elements above sodium (Na). X-ray element maps can easily be acquired on the microprobe. Using 512x512 pixel maps, up to nine element maps (5 elements on WDS, 4 with EDS), plus a backscattered electron (BSE) image, can be obtained simultaneously. Maximum resolution on any one map is one micron per pixel. Utilizing the coordinate system, maps of entire thin sections can be generated.
Mass Spectrometry Facility (Brown Chemistry) Contact: Lisa Chen	Gas Chromatograph - Mass Spectrometer (GC - MS) Agilent 5977A GC/MSD System This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass range 1050 Da).
Mass Spectrometry Facility (Brown Chemistry) Contact: Lisa Chen	Mass Spectrometer Bruker Autoflex Speed Linear (L) Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometer (MALDI-TOF MS). The Bruker autoflex Speed is a linear time-of-flight (TOF) mass spectrometer equipped with a matrix-assisted laser desorption ionization (MALDI) ion source. This instrument is routinely used for analysis of organic molecules, natural products, and macromolecules, including recombinant proteins, oligonucleotides, polysaccharides and synthetic polymers.

Good Manufacturing Practice (GMP)

Facility	Instrument/Service/Resource Unit
Roger Williams Medical Center Resources Contact: John W. Morgan, Ph.D.	Cell Immunotherapy and Gene Therapy (CITGT) Good Manufacturing Practice (GMP) Facility Services and Equipment The Cell Immunotherapy and Gene Therapy GMP facility supports the manufacture of investigational new drug (IND) products for principal investigators under FDA rules and regulations.

Facility

Instrument/Service/Resource Unit

Imaging and Molecular Biology and Nanomedicine Core

Contact: John W. Morgan, Ph.D.

Cell Immunotherapy and Gene Therapy (CITGT) Good Manufacturing Practice (GMP) Facility

Services and Equipment
The Cell Immunotherapy and Gene Therapy GMP facility supports the manufacture of investigational new drug (IND) products for principal investigators under FDA rules and regulations.

Facility	Instrument/Service/Resource Unit
Pharmaceutical Development Institute Contact: Catherine Curtin-Miller	Training Center Clinical Research Service, GMP education Providing GMP industrial experience in drug manufacturing and analytical testing for students in various related undergraduate, graduate and adult education programs, as well as industry-specific training in collaboration with pharmaceutical and biotechnology firms.
Pharmaceutical Development Institute Contact: Catherine Curtin-Miller	GMP analytical testing Clinical Research Service, GMP analytical Providing pharmaceutical testing services including turn-key stability studies
Pharmaceutical Development Institute Contact: Catherine Curtin-Miller	GMP manufacturing Clinical Research Service, GMP manufacturing Assisting academic, government and industry partners with the manufacture of solid dose forms for veterinary and human use
Pharmaceutical Development Institute Contact: Catherine Curtin-Miller	current Good Manufacturing Practice (cGMP) Facility Service Rapid expansion in the markets for biologics and small molecule pharmaceutical products has created a critical demand for skilled professionals (from equipment operators to advanced technical and quality assurance personnel) and additional infrastructure to support drug development and manufacturing. The current Good Manufacturing Practice (cGMP) Facility at the University of Rhode Island (URI) offers a state-of-the-art training, development and manufacturing facility for GMP production of solid oral dosage forms.

Histology & Pathology

Facility	Instrument/Service/Resource Unit
Molecular Pathology Core - Brown Contact: David Silverberg	Histology/Tissue Processing - Immunolabelling Service Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Immunogold, TUNEL Assay
Molecular Pathology Core - Brown Contact: David Silverberg	Paraffin Embedding Workstation Leica EG1150C Paraffin Embedding Center Paraffin embedding
Molecular Pathology Core - Brown Contact: David Silverberg	Microtome Leica RM2265 Automated Microtome Paraffin and plastic sectioning
Molecular Pathology Core - Brown Contact: David Silverberg	Cryostat Leica CM3050S Cryostat Sectioning of fixed and unfixed frozen samples
Molecular Pathology Core - Brown Contact: David Silverberg	Vibratome Leica VT 1000S Vibratome Sectioning of fixed samples
Molecular Pathology Core - Brown Contact: David Silverberg	Histology/Tissue Processing - Tissue Sample Processing and Embedding Service Paraffin Processing and Embedding- automated and manual, Plastic Processing and Embedding, TEM and SEM Processing and Embedding, Frozen Tissue-Embedding in OCT Block, Sample Preparation for Serial Blockface Imaging
Molecular Pathology Core - Brown Contact: David Silverberg	Histology/Tissue Processing - Sectioning Service Paraffin sectioning, plastic sectioning, Frozen/Cryostat prep and sectioning, TEM ultrathin sectioning, Vibratome, Serial and Step sectioning
Molecular Pathology Core - Brown Contact: David Silverberg	Histology/Tissue Processing - Staining Service Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS), Masson's Trichrome, Special Stains

Facility	Instrument/Service/Resource Unit
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Microtome Thermo Scientific Shandon Finesse 325 manual microtome With a lightly balanced flywheel it is ideal for routine paraffin sectioning.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Sample Preparation - Cutter Buehler-Isomet Cutter The IsoMet Low Speed Cutter is a precision sectioning saw designed for cutting various types of materials with minimal deformation. Its low kerf loss capability and great versatility in holding all types of sample shapes and configurations, gives today's laboratory a precision sectioning tool capable of cutting viturally any material including brittle or ductile metals, composites, cements, laminates, plastics, electronic devices and biomaterials. The Cutter is equipped with a diamond studded carborundum wheel and Buehler Ecomet 4 grinder/polisher with Automet 2 powerhead.

Facility

Instrument/Service/Resource Unit

Contact: Lei Wei, PhD

Microtome

Thermo Scientific Shandon Finesse 325 manual microtome
With a lightly balanced flywheel it is ideal for routine paraffin sectioning.

Imaging and Molecular Biology and Nanomedicine Core

Contact: Lei Wei, PhD

Sample Preparation - Cutter

Buehler-Isomet Cutter
The IsoMet Low Speed Cutter is a precision sectioning saw designed for cutting various types of materials with minimal deformation. Its low kerf loss capability and great versatility in holding all types of sample shapes and configurations, gives today's laboratory a precision sectioning tool capable of cutting viturally any material including brittle or ductile metals, composites, cements, laminates, plastics, electronic devices and biomaterials. The Cutter is equipped with a diamond studded carborundum wheel and Buehler Ecomet 4 grinder/polisher with Automet 2 powerhead.

Facility	Instrument/Service/Resource Unit
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Sample Preparation - Knifemaker LKB 7800 Knifemaker Slide/Sample Preparation
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microtome Leica RM2165 Motorized Rotary Microtome Slide/Sample Preparation
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microtome TBS Microtome plus Cryostat Slide/Sample Preparation

Facility

Instrument/Service/Resource Unit

Contact: Dr. Rebeka Merson

Sample Preparation - Knifemaker

LKB 7800 Knifemaker
Slide/Sample Preparation

Contact: Dr. Rebeka Merson

Microtome

Leica RM2165 Motorized Rotary Microtome
Slide/Sample Preparation

Rhode Island INBRE Centralized Research Core Facility (CRCF)

Contact: Dr. Rebeka Merson

Microtome

TBS Microtome plus Cryostat
Slide/Sample Preparation

Facility	Instrument/Service/Resource Unit
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Analyzer - Live Cell Roche xCELLigence Real-Time Cell Analysis System Label free live cell analyzer
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Biological Safety Cabinet NuAire, Laminar Flow Hood Cell culture facility
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Cell Counter Nexcellom Cellometer Vision Cell Counter Automated cell counter

Facility

Instrument/Service/Resource Unit

Contact: Dr. Ang Cai

Analyzer - Live Cell

Roche xCELLigence Real-Time Cell Analysis System
Label free live cell analyzer

Rhode Island INBRE Centralized Research Core Facility (CRCF)

Contact: Dr. Ang Cai

Biological Safety Cabinet

NuAire, Laminar Flow Hood
Cell culture facility

Rhode Island INBRE Centralized Research Core Facility (CRCF)

Contact: Dr. Ang Cai

Cell Counter

Nexcellom Cellometer Vision Cell Counter
Automated cell counter

Facility	Instrument/Service/Resource Unit
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Respiratory Core Service The mission of the Respiratory Core is to develop centralized systems for cardiopulmonary structural/functional assessments and mitochondrial function analysis, with the goal to enhance the productivity and the research impact of CPVB COBRE investigators and others in the state of Rhode Island. We are committed to providing reproducible, economical, and high-quality services. We offer consultation on experimental design, provide cutting edge technological approaches and high-end instrumentation, generate and analyze data.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Organ Function Core Service To provide measurement of heart, lung, and vessel function, in vivo angiogenesis, and transient gene manipulation in vivo. The COBRE Core will provide expertise and state of the art equipment for assessing heart, lung, and vessel function, in vivo angiogenesis, and transient gene manipulation in vivo. The Cell/Organ Core has been measuring heart and lung function using echocardiogram and Flexivent™ methodological approach.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Cell Isolation and Tissue Culture Core Service The function of the Cell Isolation and Tissue Culture Core is to provide efficient, economical, and reproducible isolation of pulmonary and cardiac endothelial cells, fibroblasts, vascular smooth muscle cells, epithelial cells, macrophages; propagation, and phenotypic characterization and function.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Analyzer - Cell Metabolism Seahorse Biosciences - XF Extracellular Flux Analyzer XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. XF Technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Sensing equipment - Electrical Cell Impedance Sensing (ECIS) Applied BioPhysics, Model 100 and Model ECIS Z Theta Measures cell impedance using altering currents across cell monolayers and measuring the change in electrical potential. Impedance is calculated using Ohm's law.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Evaporator Buchi RotaVapor R215 Evaporator Rotary evaporator for removing solvents.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Ventilator - Mechanical SCIREQ FlexiVent Contains a computer-controlled precision piston pump that can intersperse mechanical ventilation with a variety of volume and pressure controlled maneuvers to obtain accurate, reproducible measurement of respiratory mechanics for pulmonary research.

Imaging

Facility	Instrument/Service/Resource Unit
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Software - Analysis Software: Amira 2019 Software for image analysis and 3D visualization
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Sample Preparation - Critical Point Dryer Ladd Research Industries Removes liquid from biological specimens for SEM
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Sample Preparation - Glow Discharge Unit Emitech K100X Adds a charge to make TEM samples hydrophilic
Magnetic Resonance Imaging (MRI) Facility Contact: Michael Worden, PhD	Imaging System - Magnetic Resonance Imaging (MRI) Scanner Siemens 3 Tesla Prisma fit Brain structure and function in normal and clinical populations as well as studies of other body systems, non-invasive animal imaging and materials science.
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Multiphoton Olympus FV-1000-MPE Multiphoton Laser Scanning Microscope Deep optical sectioning of fluorescently labeled samples with SIM photoactivation
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Fluorescence Zeiss Axiovert 200M Fluorescence Imaging of samples labeled with fluorescent indicators
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Fluorescence Zeiss Axiovert 200M Fluorescence Imaging of samples labeled with fluorescent indicators
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Confocal Olympus FV3000 Confocal Microscope Optical sectioning of samples labeled with fluorescent indicators. Equipped with a resonant scanner for fast optical sectioning
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Confocal Zeiss LSM 710 Confocal Optical sectioning of samples labeled with fluorescent indicators
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Fluorescence Zeiss Lumar Stereo Fluorescence Imaging of samples labeled with fluorescent indicators
Mouse Transgenic and Gene Targeting Facility Contact: Jinping Luo, PhD	Microscope Nikon Eclipse TE200 Inverted Microscope Transgenics and gene targeting microinjection microscope
Mouse Transgenic and Gene Targeting Facility Contact: Jinping Luo, PhD	Microscope Nikon Eclipse TS100 (cell culture scope) Transgenics and gene targeting cell culture scope
Mouse Transgenic and Gene Targeting Facility Contact: Jinping Luo, PhD	Microscope Nikon SMZ1500 Zoom Stereomicroscopes Transgenics and gene targeting dissecting microscopes (2 units)
Mouse Transgenic and Gene Targeting Facility Contact: Jinping Luo, PhD	Microscope Nikon SMZ800 Zoom Stereomicroscopes Transgenics and gene targeting surgical microscope
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Electron Thermo Apreo VS Scanning Electron Microscope (SEM) for serial block-face imaging Equipped for standard scanning electron microscopy as well as serial-block face imaging. The automated system is capable of imaging large z-stacks at isotropic 10x10x10 nm resolution. The series of images can be processed for 3D reconstruction of cells and tissues, with sufficient resolution to identify small vesicles, organelles and neural connections.
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Electron Philips 410 Transmission Electron Microscope (TEM) Transmission electron microscopy with Advanced Microscopy Techniques (AMT) Camera Accessory
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microtome Reichert Ultramicrotome Ultrathin sectioning for light microscopy and transmission electron microscopy
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microtome Diamond Knife Set Diatome DKS Used on Reichert microtome to cut ultrathin sections for TEM
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Sample Preparation - Sputter Coater System Emitech K550 Covers an EM specimen with a thin layer of conducting material
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Training and Technical Assistance - Bioimaging Service Service to provide Bioimaging technical assistance and training
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Imaging System - Gel Imager LI-COR Odyssey Clx IR (Infrared) Imaging System Western blot analysis, EMSA, protein arrays, In-Cell Western Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation, and tissue section analysis.
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Fluorescence Nikon Ti2-E Fluorescence Microscope Motorized fluorescence microscope with Perfect Focus System and software for high-content screening and 2D/3D deconvolution.
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microtome Leica EM UC7 Ultramicrotome Biological TEM and SBF prep
Molecular Pathology Core - Brown Contact: David Silverberg	Microscope Nikon Eclipse 50i light microscope Collaborative analyses of histological slides and digital imaging capability
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Confocal Zeiss LSM 800 Confocal Optical sectioning of samples labeled with fluorescent indicators
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Imager and Microplate Reader BioTek Cytation 5 Cell Imaging Multi-Mode Reader UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence. Cell migration and invasion assays, Live cell imaging, Fluorescence cell imaging (DAPI & GFP), Multi-mode reader applications, Immunofluorescence, Phenotypic assays, Histology
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Fluorescence Nikon Ti2-E Fluorescence Microscope for high-content imaging The Nikon Ti2-E fluorecence microscope is a high-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates). The microscope includes 'Perfect Focus' for autofocus applications, a Photometrics Prime 95B sCMOS camera for imaging fluorescence, a 16 megapixel Nikon DS-Ri2 color camera for imaging histology slides, a motorized stage and NIS-Elements High Content (HC) software for multiwell plate acquisition.
Electron Microscopy Facility Contact: Anthony McCormick	Microscope - Focused Ion Beam Electron (FIB) Thermo/FEI Helios Dual Beam Focused Ion Beam Electron Microscope (FIB) with an Omniprobe nanomanipulator and autoTEM sample preparation The FIB is fully equipped for (near) simultaneous ion/electron beam imaging and patterning of specimens. A Pt metal deposition source is available for ion (or electron) beam assisted deposition of conducting lines. A second source is available to enhance the carbon removal rate. An Omniprobe AutoProbe 200.2 allows the physical manipulation of samples with nanometer precision and is used along with Pt metal deposition and standard FIB trenching to allow the selection and removal of electron transparent TEM samples from specific microstructural features. A NPGS pattern generation system (plus beam blanker and external scan interface) controls the electron beam for high resolution, interlaced pattern generation used in the FIB fabrication of nanostructures. Three dimensional crystallographic data can be generated from specimens using the fully automated EBSD system (Oxford HKL&EBS-3). The microscope is also equipped with an EDS detector for elemental analysis. An electron flood gun can be used with the ion beam to image/etch nonconductive samples. A low temperature holder is available for beam sensitive samples.
Electron Microscopy Facility Contact: Anthony McCormick	Microscope - Scanning Electron (SEM) LEO 1530 High Resolution Scanning Electron Microscope (HRSEM) The LEO 1530 VP ultra-high resolution field emitter SEM that allows for a resolution of 1 nm at a voltage of 20 kV in high vacuum and 2 nm at 30 kV in the variable pressure mode. It is fully equipped with EDS for chemical mapping and includes a Debbem tensile test stage 5kn. The instrument is equipped with Nabity pattern generating software a high speed beam blanker and is set-up for high resolution electron-beam lithography. The LEO 1530 VP is capable of a resolution of 2.1 nm at very low electron energies (1 kV) which is of great use for beam-sensitive materials like polymers and insulating surfaces. Also very useful for insulating samples is the variable operating pressure mode which allows insulating materials to be imaged without coating the surface.
Electron Microscopy Facility Contact: Anthony McCormick	Microscope - Transmission Electron (TEM) FEI CM20 TEM The FEI CM20 is available for applications that require high tilt, moderate resolution (0.23 nm). This 200 kV analytical TEM allows the large specimen tilts needed for dislocation characterization and weak-beam imaging applications. It is equipped with a low background double tilt holder and a liquid nitrogen temperature holder. Compositional analysis is performed on this instrument using energy dispersive x-ray spectroscopy. Images are recorded using a large field of view, side mounted, digital image acquisition system.
Electron Microscopy Facility Contact: Anthony McCormick	Microscope - Scanning Transmission Electron Microscope -S(TEM) JEOL 2100F - S (TEM) The 2100F is a 200 kV, field emission source, high-resolution/analytical S(TEM) capable of 0.10 nm lattice resolution. It is primarily used for obtaining lattice images or when chemical or crystallographic information is required from laterally small areas (1-10 nm). The instrument is equipped with an x-ray spectrometer capable of light element detection, a Gatan image filter (GIF) for element and phase analyses and enegy filtered imaging, a high tilt holder for electron tomography and a high-resolution CCD camera system for digital image acquisition and processing. A low background double-tilt holder for chemical analysis by EDS, a standard double tilt holder, a heating holder capable of 1000C, and a heating straining holder are available. Probes as small as 1 nm in diameter are accessible for micro-diffraction experiments. STEM mode for EDS line and area scans, bright and dark field STEM detectors, and high angle annular dark field is capable in STEM mode.
Electron Microscopy Facility Contact: Anthony McCormick	Spectrometer - ThermoElectron K-Alpha XPS Thermo/FEI K-Alpha X-Ray Photoelectron Spectrometer This equipment is used to study the surface composition of materials using photoelectrons generated by 30 kV monochromated Aluminum K-Alpha x-rays hitting the sample. It is equipped with an Argon sputter gun for surface cleaning and depth profiling. There is a tilt and rotate holder. There is a transfer holder so samples can be transferred to the XPS with exposure to atmosphere.
Electron Microscopy Facility Contact: Anthony McCormick	Microscope - Quattro S-E(SEM) Thermo/FEI Quattro S-E(SEM) The Quattro is an environmental SEM which means that the sample chamber can be set to a (relatively) high pressure for water containing samples.Â This SEM has EDS (X-ray detection) and EBSD (electron backscatter). The Quattro S is a field emission scanning electron microscope capable of generating and collecting all available information from any type of sample material. It provides the most versatile high-resolution low-vacuum FEG SEM with extended low-vacuum capabilities and the most versatile high resolution SEM for characterization, prototyping and in situ analysis. The features include three vacuum modes enabling investigation of conductive, non-conductive and high-vacuum incompatible materials such as High-vacuum mode (<6*10-4 Pa) for imaging and microanalysis of conductive and/or conventionally prepared specimens; Low-vacuum mode (10 to 130 Pa) for imaging and microanalysis of non-conductive specimens without preparation; and ESEMâ„¢ mode (10 to 4000 Pa) for high-vacuum incompatible specimens which are impossible to investigate with traditional EM methods. The system is equipped with a 5-axes motorized x-y-z-tilt-rotate stage,for Sample navigation.
Electron Microscopy Facility Contact: Anthony McCormick	Sample Preparation Electron Microscope Facility Sample Preperation Area Tools available in the EMF sample preparation area include a jet polisher, polishing benches, dimpler and high and low speed diamond saws.The sample prep area has a carbon thread evaporator and Gold/Palladium sputter coater for making conductive coatings on SEM samples. There is an ion mill (PIPS) for making TEM samples. There is also a plasma cleaner for TEM sample holders and SEM samples.
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Confocal Zeiss LSM 880 Confocal Optical sectioning of samples labeled with fluorescent indicators
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Microplate Reader BioTek Synergy H1 Hybrid Multimode (H1MM) Microplate Reader UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence. Cell based assays, Biomarker assays, Micro-volume analysis of nucleic acid and protein, Kinetic assays.
Behavior and Neuroimaging Core Contact: Michael Worden	Training and Technical Assistance - Support Clinical Research Resources, Behavior and Neuroimaging The mission of the Behavior and Neuroimaging Core (BNC) is to provide a coherent set of resources that will facilitate the implementation of each of the COBRE Project Leader’s research aims. The BNC provides ongoing support, training, assistance and advice to COBRE project leaders in the practical aspects of data collection, data management and data processing across a wide variety of neuroscience related methodologies. The BNC also serves as a resource to the broader neuroscience community at Brown and its affiliated hospitals and the broader Rhode Island academic community by providing formal and informal training, accessible data processing pipelines, and documentation of these and related resources. The support provided by the BNC is distinct from but complementary to services provided by the Design and Analysis Core (DAC). Whereas the DAC is focused primarily on study design, analytical modeling, and techniques for inference, the focus of the BNC is on practical aspects of research conduct such as efficient and high-quality data acquisition, implementation and deployment of standard and newly developed tools for analysis of large neuroimaging datasets, and lowering barriers to the use of such tools through direct assistance, training and documentation. Because the missions of the BNC and DAC are highly complementary, the effectiveness of each core is strengthened by collaborative relationships between them. The analytical techniques developed by the DAC in partnership with the Project Leaders are predicated upon robust high-quality data. Similarly, realization of study design principles requires practical expertise with the tools necessary to implement those principles. The BNC and DAC will share a full-time staff member with expertise in research design, statistical modeling, data management and high-performance computing.
Behavior and Neuroimaging Core Contact: Michael Worden	Training and Technical Assistance - Facilitate Experimental Implementations Clinical Research Resources, Behavior and Neuroimaging Control experiments, Eye tracking, EEG guidance
Behavior and Neuroimaging Core Contact: Michael Worden	Training and Technical Assistance - MRI Assistance Clinical Research Resources, Behavior and Neuroimaging Design of experiments, Analysis pipelines, EEG in the MRI scanner, Timing verification
Behavior and Neuroimaging Core Contact: Michael Worden	Data Storage - Generalized Solutions Clinical Research Resources, Behavior and Neuroimaging xNAT database, Containers for data presentation, Beyond the desktop with VMs
Behavior and Neuroimaging Core Contact: Michael Worden	Training and Technical Assistance - High Performance Computing (HPC) Clinical Research Resources, Behavior and Neuroimaging Assistance with High Performance Computing (HPC)
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Design - Experimental Design Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Multilevel and Hierarchical Modeling Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Machine Learning Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Feature Extraction Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Effective and Functional Connectivity Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Spatial-Temporal Modeling Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Time Series Modeling Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	General Linear Model (GLM) and Linear Model (LM) Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Analysis of Variance (ANOVA) Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Regression for High Dimensional Data Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Analysis - Network Analysis Clinical Research Resources, Behavior and Neuroimaging
Design and Analysis Core Contact: COBRE_CNSF@brown.edu	Causal Interference Clinical Research Resources, Behavior and Neuroimaging
In Vivo Optical Imaging Core (Legorreta Cancer Center at Brown University) Contact: Arunasalam Navaraj, PhD	Training and Technical Assistance Service Advise investigators with experimental design for in vivo imaging applications, provide access to instrumentation and training. Assist investigators with in vivo mouse, organ or cellular imaging, probe selection, antibody labeling, image analysis and presentation. Provide support for grant submissions.
X-ray Reconstruction of Moving Morphology Facility (XROMM) Contact: Beth Brainerd, PhD	Imaging System - MicroComputed Tomography (uCT) Scanner Bruker BioSpin SkyScan 1276 in vivo microCT scanner for small animals (mouse to rat size)
Leduc Bioimaging Facility Contact: Robbert Creton, PhD	Microscope - Slide Scanner Olympus VS200 Automated slide scanner for imaging fluorescence and brightfield (e.g. H&E slides).

Facility	Instrument/Service/Resource Unit
Bryant Unistructure Science Spaces Contact: Christopher Reid	Sample Preparation - Sputter Coater System Denton Vacuum Desk V gold sputter coater and carbon coater Coat non-conducting samples with gold or carbon for use on the SEM
Bryant Unistructure Science Spaces Contact: Christopher Reid	Microscope Zeiss Primo Star with AxioCam Recordable imaging
Bryant Unistructure Science Spaces Contact: Christopher Reid	Microscope Zeiss Axiovert 40CFL Inverted Microscope Recordable inverted microscopy
Bryant Unistructure Science Spaces Contact: Christopher Reid	Microscope Zeiss A1 AXIO Polarized Light Microscope and Q imaging RETIGA 400R Imager Recordable polarized light imaging
Bryant Unistructure Science Spaces Contact: Christopher Reid	Microscope - Electron JEOL JSM 6010LA Scanning Electron Microscope (SEM) Scanning electron microscope with EDS
Bryant Unistructure Science Spaces Contact: Christopher Reid	Sample Preparation - Critical Point Dryer Tousimis Sandri-PVT-3D Critical Point Dryer (CPD) Maintain structural integrity for soft tissue use of the SEM

Facility	Instrument/Service/Resource Unit
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microscope Leica MZ FLIII Stereo Fluorescent dissection microscope Dissection microscope with Leica color camera DC300F.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microscope Nikon 80i Upright Fluorescence Microscope Upright microscope with color CCD camera Spot Insight 2
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microscope Nikon TE2000E Inverted fluorescence, A live cell imaging microscope, with cooled CCD camera Roper CoolSnap HQ
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microscope Zeiss AxioImager M2 Upright Microscope, Upright microscope with color CCD camera AxioCam MRC.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Centrifuge - Ultra Beckman Coulter Optima XPN-80 Ultracentrifuge SW-32.1Ti. SW-41Ti. TY-70.1Ti, TY70Ti rotors
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microscope - Spinning disk Andor CSU-W1 spinning disk confocal system The Spinning disk confocal microscope is a Nikon Ti-E (inverted) with Perfect Focus and motorized stage, with an Andor Zyla 4.2 plus SCMOS camera, an Andor CSU-W1 spinning disk with 405, 488 and 561 nm laser lines, as well as a SpectraX LED widefield light source and controlled by NIS-Elements software. This system is suitable for fast confocal imaging and live cell imaging, with low phototoxicity and long term focus stability. A stage incubator with CO2 and temperature control is available for maintenance of physiological conditions during live cell imaging.

Facility	Instrument/Service/Resource Unit
Roger Williams Medical Center Resources Contact: John W. Morgan, Ph.D.	Imaging Core Zeiss LSM 700 confocal, Zeiss Axioplan 2 optical, Zeiss Axiovert 200M optical, IVIS Model 100 The Imaging Core facility provides technical and logistical access for fixed and live cell fluorescence imaging via visual and digital fluorescent microscopes. This core also offers the ability to visualize fluorescent and bioluminescent markers in live animals via an IVIS imager.

Facility

Instrument/Service/Resource Unit

Contact: John W. Morgan, Ph.D.

Imaging Core

Zeiss LSM 700 confocal, Zeiss Axioplan 2 optical, Zeiss Axiovert 200M optical, IVIS Model 100
The Imaging Core facility provides technical and logistical access for fixed and live cell fluorescence imaging via visual and digital fluorescent microscopes. This core also offers the ability to visualize fluorescent and bioluminescent markers in live animals via an IVIS imager.

(Independent Nonprofit)

Facility	Instrument/Service/Resource Unit
Mansfield Bio-Incubator Contact: Alexander Margulis, PhD	Luminometer Digene DML 2000 luminometer The DML 2000 instrument is a luminometer that automates amplified chemiluminescent signal detection and results reporting for Hybrid Capture 2 (HC2) assays,

Facility

Instrument/Service/Resource Unit

Scientific Illustration and Visual Design

Contact: Alexander Margulis, PhD

Luminometer

Digene DML 2000 luminometer
The DML 2000 instrument is a luminometer that automates amplified chemiluminescent signal detection and results reporting for Hybrid Capture 2 (HC2) assays,

Facility	Instrument/Service/Resource Unit
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Imaging System - Gel Imager Bio-Rad Molecular Imager VersaDoc Quantitative digital imaging system for single or multi-color images. Includes multiplexed samples (1-D or 2-D gels), chemiluminescent and chemifluorescent blots, and microplates.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Imaging System - Gel Imager LI-COR Odyssey Classic Infared Imaging system This near-infared fluorescent detector can be used for quantitative western blots, gel shift assays, coomassie stains, tissue section and small animal imaging.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Imaging System - Gel Imager UVP GelCam 310 Bioimaging digital gel documentation system High resolution 2.0 GelCam 310 CCD with manual zoom lens for fluorscent and colorimetric gel imaging. EtBr filter included.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Microscope Nikon Eclipse TS-100F Inverted Microscope This phase contrast microscope used for tissue culture is also equipped with FITC and TRITC filters to capture fluorescent images in SPOT imaging software.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Microscope Nikon Eclipse 90i Upright Research Microscope This microscope is equipped with two cameras (color and monochromatic) and is ideal for brightfield and/or epi-fluorescent images of whole tissues etc with 4x, 10x, 20x and 40x dry objectives. The filter cubes available are DAPI, FITC, and TRITC.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Imaging System PerkinElmer FMT 4000 Fluorescence Tomography System. The FMT 4000 fluorescence tomography imaging system provides the greatest utility of the FMT Systems with the ability to quantitate up to four fluorophores simultaneously. The system comes with four excitation laser channels (635, 680, 750, and 790 nm) and is useful for a range of applications including oncology, inflammation, and cardiovascular disease. It is ideal for researchers who are well-versed in 3D fluorescence tomography and require the widest range of in vivo applications and imaging agents.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Imaging System Faxitorn Pre-clinical imaging system Ultra-high resolution x-ray imaging is an important tool in investigating a variety of disease states. From metastatic cancer to arthritis and osteoporosis, small animal models are commonly used. Both in vivo and ex vivo planar imaging is used to screen, track, and evaluate structural changes in these studies. Cabinet x-ray systems allow researchers to take images directly in the vivarium to protect the integrity of the colony. These planar imaging systems are less expensive, subject animals to lower doses, and provide faster results than microCT systems.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Microscope Olympus SZ61 Zoom Stereomicroscope Greenough design with two optical paths inclined at an inward angle. Magnification range 0.67X to 4.5X; zoom ratio 6.7:1; FN 22. Working distance of 100mm; observation tube angle 45 degrees; right and left eyepiece tubes interlocked; interpupillary distance adjustment 50-76mm; forward positioned zoom adjustment knobs with zoom magnification graduations. With 0.5x cmount for documentation.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Imaging System Xtreme back illuminated 4MP with x-ray for small animal imaging High sensitivity luminescence, fluorescence, radioisotopic, and radiographic imaging for up to 5 mice at a time. Isofluarne and temperature controlled chamber for the mice.
Bioengineering Core Contact: Braden Fleming, PhD	Imaging System - Camera Qualisys Oqus 5-Series The Oqus cameras are designed to capture accurate mocap data with very low latency and works with both passive and active markers.
Bioengineering Core Contact: Braden Fleming, PhD	Microscope Asylum Research MFP-3D-BIO Atomic Force Microscope
Bioengineering Core Contact: Braden Fleming, PhD	Microscope Nikon TiU inverted microscope Inverted Microscope

Facility	Instrument/Service/Resource Unit
Animal Models Core Contact: Gerard Nau, MD	Imaging System - fluorescent and bioluminescent PerkinElmer IVIS Lumina III in vivo imaging system The IVIS Lumina III is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 26 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s optional high resolution short cut off filters.

Facility

Instrument/Service/Resource Unit

Animal Models Core

Contact: Gerard Nau, MD

Imaging System - fluorescent and bioluminescent

PerkinElmer IVIS Lumina III in vivo imaging system
The IVIS Lumina III is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 26 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s optional high resolution short cut off filters.

Facility	Instrument/Service/Resource Unit
PC - Albertus Magnus Hall Labs Contact: Charles Toth	Imaging System - Camera Fastec HiSpec1, Low-Light High-Speed Camera Innovative Low-Light High-Speed Camera. Monochrome with 2GB Memory, C-Mount, includes ImageBLITZ Auto Trigger, Burst Trigger Mode, Multi- Sequence Record Mode (1280 x 1024 at 506fps) 2 Units
PC - Albertus Magnus Hall Labs Contact: Charles Toth	Microscope - Electron Philips EM-300 Transmission Electron Microscope (TEM) Transmission electron microscopy
PC - Albertus Magnus Hall Labs Contact: Charles Toth	Sample Preparation - Laboratory - Prep Lab Electron Microscopy Prep Lab Laboratory space for preparing samples for electron microscopy
PC - Albertus Magnus Hall Labs Contact: Charles Toth	Microscope - Electron Amray-1000 Scanning Electron Microscope (SEM) Scanning Electron Microscope equipped with EDEX for elemental analysis and a digital imaging system, a Zeiss Axiophot equipped for bright field, dark field, DIC and fluorescence microscopy with a digital imaging system
PC - Sowa Hall Labs Contact: Charles Toth	Microscope Zeiss LMS 700 Flexible Confocal Microscope Performs fluorescence measurements, creates optical sections of samples and combines them into 3D image stacks.

Facility	Instrument/Service/Resource Unit
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope American Optical Series 10 Fluorescence Microstart Microscopy course
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope Nikon E600 Epifluorescence with DIC/Phase and digital camera Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope Olympus CKS41 Inverted Microscope Tissue culture general use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope Olympus Compound Light Microscope General use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope Olympus FluoView 1000 Laser Scanning Confocal Microscope Research and Microscopy course
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope Olympus Inverted Epifluorescence Microscope with digital camera Tissue culture/Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microscope Zeiss SteREO discovery V8 Microscope with Digital Camera Research

Facility	Instrument/Service/Resource Unit
RISD Nature Lab Contact: Jennifer Bissonnette	Microscope Phenom G2Pro Scanning Electron Microscope (SEM) Desktop Scanning Electron Microscope with ProSuite tiling and remote access software
RISD Nature Lab Contact: Jennifer Bissonnette	Microscope Olympus SZX16 Stereomicroscope Light microscope with 12.5 MP Jenoptik progressive scan camera, computer workstation, graphics and imaging software
RISD Nature Lab Contact: Jennifer Bissonnette	Microscope Olympus IX51 Inverted Fluorescent/Brightfield Microscope Fluorescence/brightfield microscope with 12.5 MP Jenoptik progressive scan camera, computer workstation, graphics and imaging software
RISD Nature Lab Contact: Jennifer Bissonnette	Microscope Olympus CH30 Upright Light Microscope Upright light microscope with 5 MP Jenoptik digital camera, Canon Vixia HFS20 HD video camera, computer workstation, graphics and imaging software (2 units)
RISD Nature Lab Contact: Jennifer Bissonnette	Microscope Olympus SZ40 Stereomicroscope Light microscope with 5 MP Jenoptik digital camera, computer workstation, graphics and imaging software
RISD Nature Lab Contact: Jennifer Bissonnette	Microscope Olympus SZ30 Stereomicroscope Light microscope with LED illuminator (24 units)

Facility	Instrument/Service/Resource Unit
Marine Science Research Facility Contact: Dawn Outram	Imaging System - Camera Olympus EP50 Microscope Camera w/ HDMI Monitor Captures Microscope video and still images (C-mount)
Marine Science Research Facility Contact: Dawn Outram	Analyzer - Fluid Particle Counter and Imager Fluid Imaging Technologies FlowCam Imaging Particle Analysis: counts and records images of particles in liquid samples
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Imaging System - Gel Imager LI-COR Odyssey IR (Infrared) Imaging System IR gel imager
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Microplate Reader Molecular Devices SpectraMax M2 Fluorescence/UV/VIS
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Microplate Reader - Luminometer Promega GlowMax System Chemiluminescence
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Microscope Nikon Eclipse E600 Upright Microscope Microscope
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Microscope Nikon Eclipse Ti2 inverted confocal microscope Confocal Microscope
Marine Science Research Facility Contact: Dawn Outram	Microscope Nikon inverted Microscope Microscopy. Works with Olympus EP50 Camera
Marine Science Research Facility Contact: Dawn Outram	Microscope Olympus SZX16 Stereo Microscope Microscopy. Works with Olympus EP50 Camera
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - X-ray Zeiss Xradia Versa 610 with in situ tensile, compression and temperature stage X-ray microscopy and tomography of samples up to 10 cm in diameter with resolution up to 500 nm
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Confocal Raman Spectroscopy WITec alpha 300R Confocal imaging and chemical analysis by Raman spectroscopy up to 100x magnification, 3D chemical imaging, large area imaging, principal component analysis of chemical composition, upright and inverted mode, automated XY stage, 785 and 532 nm excitation lasers
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microtome Sorvall MT2-B ultramicrotome Preparation of biological tissue, cross sections and embedded samples for TEM.
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Sample Preparation - Sputter Coater System Cressington 7002-8 sputter coater with film thickness monitor Coat non-conducting samples with gold for use on the SEM, rotating/tilting stage for coating of large specimens.
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Spectrometer - Energy Dispersive X-Ray Fluorescence Shimadzu EDX-8100 Energy dispersive X-ray fluorescence of powders, solids and liquids. Ambient pressure, vacuum and He atmosphere
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Field Emission-Scanning Electron Microscope (FE-SEM) Zeiss Gemini Sigma VP Field Emission-Scanning Electron Microscope (FE-SEM) Field emission scanning electron microscope Zeiss Gemini Sigma VP, with cryoSEM (Gatan), energy dispersive X-ray spectroscopy (Oxford Instruments), variable pressure mode, backscattering detector, in lens detector and secondary electron detector
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Scanning Transmission Electron JEOL F200 Scanning transmission electron microscope with cryogenic capabilities, direct electron camera (Gatan K3), Energy dispersive X-ray spectroscopy (JEOL), electron energy loss spectroscopy (Gatan). Sub nanometer resolution, 80 and 200 kV operation, TEM, STEM and diffraction
Cell Analysis and Sorting Core Contact: Alan Rothman, M.D., Research Professor	Microplate Reader BioTek Cytation 3 Imaging plate reader; 5 colors (brightfield, GFP, RFP, DAPI, Cy5); 10x, 20x, 60x objectives
Cell Analysis and Sorting Core Contact: Alan Rothman, M.D., Research Professor	Microscope Life Technologies Evos FL, Fluorescence Microscope Fluorescence microscope; 4 colors (GFP, RFP, DAPI, Cy5); 10x, 40x, 100x objectives
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Transmission Electron Microscope JEOL JEM-2100 LaB6 Transmission Electron Microscope (TEM) Transmission electron microscope JEOL JEM 2100 with sub nanometer resolution, cryoTEM (Gatan) and energy dispersive X-ray spectroscopy (Oxford Instruments)
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Fourier Transform Infrared Spectroscopy Shimadzu AIM-9000 with Shimadzu IRTracer100 FTIR microscope and spectrometer, ATR, liquid and gas cell.
Marine Science Research Facility Contact: Dawn Outram	Microscope Nikon Eclipse Ni epifluorescence microscope Epifluorescence and phase microscopy with digital camera system
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Imager - Biomolecular Azure Sapphire Biomolecular Imager The Azure Sapphire Biomolecular Imager is a laser scanning system has four lasers (488, 520, 658 and 784 nm) giving a wide range of excitation. The instrument supports long and short wavelength Near IR fluorescence, red/green/blue imaging, and chemiluminescent imaging. Multiple detectors are used depending on the excitation wavelength and application for maximum sensitivity. It can accommodate gels up to 25cm by 25 cm.
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - High Content Screening System Perkin Elmer - Opera Phenix High Content Screening System: Automated confocal fluorescence microscope designed for high-throughput 3D imaging of fluorescent samples in microplates, live-cell imaging, drug screening, spheroid imaging
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Sample Preparation - Critical Point Drier Tousimis Sandri-PVT-3B Critical point dryer (CPD) Maintain structural integrity for soft tissue use of the SEM.
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Microscope - Atomic Force Shimadzu SPM-9700 Scanning probe microscope for atomic force microscopy, magnetic force microscopy, liquid cell analysis, Kelvin force microscopy, I/V curves, nanomechanical measurements

Facility	Instrument/Service/Resource Unit
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Imaging System - Slide Scanner Aperio ScanScope CS Digital Slide Scanner CS Brightfield 5 slide scanner with two workstations with Aperio brightfield imaging analysis software.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Imaging System - Slide Scanner Aperio Scan Scope FL Fluorescent Digital Slide Scanner 5 slide scanner with Indica Labs fluorescence imaging analysis software.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Imaging System - Echocardiograph VisualSonics, Vevo 2100 Imaging System A high-frequency, high-resolution digital imaging platform with linear array technology and Color Doppler Mode. The Vevo 2100 Imaging System enables in vivo visualization, assessment, and measurement of anatomical structures and hemodynamic function in longitudinal imaging studies for small animal and zebrafish phenotyping.
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Microscope Nikon Ni-E upright research microscope Capture both phase, DIC, brightfield and fluorescence images at high magnification with enhanced features such as Z-stack imaging and multi-channel
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Imaging system - Laser Doppler Moor instruments, laser Doppler imaging system Laser Doppler Imaging examines blood flow non-invasively. Used to examine blow flow in capillary beds in response to pulmonary hypertension, exercise, and/or ischemia/reperfusion.

Imaging/Studio Production

Facility

Instrument/Service/Resource Unit

Contact: Vinald Francis

Design - Illustration, Graphic Design, and Data Displays

Service
BioMedical Communications offers medical and scientific illustration, graphic design, and data displays for use in web, digital, and print media to faculty across the Division of Biology and Medicine.

Facility	Instrument/Service/Resource Unit
RISD Media Resources Contact: David Chandler	Imaging System - Audio\|Visual Kodak zi 10 Video Camera (8 units) Portable HD pocket camcorder, batteries, adaptor cables, cases, accessories
RISD Media Resources Contact: David Chandler	Imaging System - Audio\|Visual H4N ZOOM Audio Recorder (5 Units) High performance portable digital audio recorder, batteries, adaptor cables, cases, accessories
RISD Media Resources Contact: David Chandler	Imaging System - Audio\|Visual ZOOM Q3 Video Camera (7 Units) Portable palm camcorder, batteries, adaptor cables, cases, accessories
RISD Media Resources Contact: David Chandler	Imaging System - Audio\|Visual EPS-SonyPMWEX1R-01 (2 Units) High performance HD CMOS solid state video camera, batteries, portable hard drive, adaptor cables, cases, studio lighting equipment, accessories

Facility	Instrument/Service/Resource Unit
MakerspaceURI Contact: makerspace@etal.uri.edu	Imaging System - 3D Scanner EinScan Pro 2X
MakerspaceURI Contact: makerspace@etal.uri.edu	Computer - Tablets IPad 7th Gen (4), Microsoft Surface
MakerspaceURI Contact: makerspace@etal.uri.edu	Imaging System - 3D Printer Makerbot Replicator+ (2); Dremel 3D20 (9); TAZ Workhorse; Formlabs (2)
MakerspaceURI Contact: makerspace@etal.uri.edu	Cutter - Vinyl GCC Expert Pro Vinyl Cutter, Cameo Silhouette (2)
MakerspaceURI Contact: makerspace@etal.uri.edu	Vacuum Former Vaquform DT2
MakerspaceURI Contact: makerspace@etal.uri.edu	Imaging System - Audio\|Visual - VR Headsets HTC Vive Pro (3); Oculus Quest 2 (2)
MakerspaceURI Contact: makerspace@etal.uri.edu	Imaging System - Camera Sony Alpha a6400 Mirrorless Digital Camera (with a tripod and accessories)

Informatics

Facility	Instrument/Service/Resource Unit
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Analysis - Data Analysis Service The core provides assistance in experimental design and data processing pipelines for high-throughput datasets generated in individual projects, particularly for DNA/RNA sequencing data.
Biomedical Informatics Core Contact: Neil Sarkar, PhD	Biomedical Informatics Services Clinical Research Resources, Biomedical Informatics Data Requests Storage and management Processing and analysis Submission and dissemination Software and Applications Requests Development REDCap Studies/Projects Project setup Education 5x5 Clinical Informatics elective Biomedical Informatics Learning Series Grant Support
Biomedical Informatics Core Contact: Neil Sarkar, PhD	Biomedical Informatics Resources Clinical Research Resources, Biomedical Informatics Cohort Identification i2b2 tranSMART [local] i2b2/SHRINE [regional] TriNetX [national] Data Collection and Management Statewide REDCap instance Wrike and SPARCRequest Data Access and Use Unified Data Sharing & Analysis (URSA) Initiative URSA stronghold URSA data requests URSA governance URSA computational tools Subject Recruitment Research Networking and Resources
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Analysis - Standardized Data Service Develop/Implement Bioinformatic Pipelines; GATK whole exome, Cut and run. Develop/Implement Standardized QC for Workflows. Experimental Design Support; Power Analysis for Microbiome and RNAseq. Exploratory Data Analysis; RNAseq, scRNA seq.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Tooling Software - R-Qckit A comprehensive quality control R package for Next Generation Sequencing FASTQ data.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Tooling Software - RSeqAn Wrappers for the SeqAn library for use in R with Rcpp
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Tooling Software - Bioflows A bioinformatics workflow engine that streamlines the most common analysis pipelines. Key features include: 1) Provision of standardized workflows 2) A module for automating SRA downloads and 3) External tools are made available as CONDA packages along with the tool.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Tooling Software - Scripts Access Genbank, BioCookBook.jl
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Tooling Containerization with Docker - NMRDock NMRdock is a Docker container image for processing raw NMR time domain data into frequency domain spectra and analyzing the spectra. The image includes NMRPipe and NMRFAM-Sparky for processing and analysis, respectively. NMRdock can be installed on any operating system (OSX, Windows, or Linux) and has the full functionality of both NMRPipe and NMRFAM-Sparky. For guides on processing BioNMR data in NMRdock, please access our Walkthrough Section
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Tooling Software- Ingenuity Pathway Analysis The CBC provides access to the Ingenuity Pathway Analysis web server at Brown University.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Analysis - Data Analysis Service The CBC now allows for a very restricted schedule of Fee-For-Service project.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Data Management CCV - RefChef RefChef is a reference management tool used to: (1) document the exact steps undertaken in the retrieval of genomic references; (2) maintain the associated metadata; (3) provide a mechanism for automatically reproducing retrieval and creation of an exact copy of genomic references.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Training and Support Service Workshops
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Training and Support Service - Consulting The Core provides two distinct complimentary services to all researchers at Brown. Appointment Based, A team of experienced computational biologists and biostatisticians are on-hand to help investigators design experiments. Weekly Consulting Sessions, The Core offers two hours walk-in Consulting sessions for all researchers to discuss any immediate issues in data analysis including trouble-shooting code, help with statistical analysis and discussion of options for bioinformatic analysis.
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Training and Support Service -Talent Development Student worker/interns, Guidance and mentoring
Computational Biology Core (CBC) Contact: Ashok Ragavendran	Training and Support Service - Outreach CBC hosts workshops, publishes tutorials on computing tools used by bioinformaticians, and provides analysis support to the Brown community.

Facility

Instrument/Service/Resource Unit

Rhode Island INBRE Molecular Informatics Core (MIC)

Contact: Christopher Hemme

Bioinformatics Analysis

Service - Bioinformatics
We provide bioinformatics consultation and services to the Rhode Island INBRE community and to the general scientific community of Rhode Island. In addition to traditional bioinformatic services, our Core is expanding to 3D/VR/AR visualization services in conjunction with the URI 3D Visualization Laboratory. We are currently developing 3D/VR visualization apps for a variety of platforms including smartphones, Vive/SteamVR, and others. We also have access to the 3D printer at the URI 3D Visualization Core for printing of marcomolecules at a reasonable price.

Marine Science

Facility

Instrument/Service/Resource Unit

PC - Albertus Magnus Hall Labs

Contact: Charles Toth

Aquatic System

Aquatic Habitats Glass Tank System and Life Support Controller
Glass tank system and life support controller with low water level protection (18-10 gallon tanks)

Facility

Instrument/Service/Resource Unit

Single Cell Technologies Core (Legorreta Cancer Center at Brown University)

Contact: Dr. Rebeka Merson

Aquatic System - Wet Lab / Aquarium room

Aquaria and related equipment
Research

Facility

Instrument/Service/Resource Unit

RISD Nature Lab

Contact: Jennifer Bissonnette

Aquatic System

Marine Ecological Habitats Closed-System Aquarium
560 L closed-system recirculating marine holding tanks with temperature control (2 units)

Facility	Instrument/Service/Resource Unit
Marine Science Research Facility Contact: Dawn Outram	Analyzer - Dissolved Gas Bay Instruments Membrane Inlet Mass Spectrometer- Dissolved Gas Analyzer Mass Spectrometer: Quantifies dissolved gases
Marine Science Research Facility Contact: Dawn Outram	Analyzer - Multiparameter Water Analyzer YSI Professional Plus Hand Held Multiparameter Water Meter Measures temperature, salinity, dissolved oxygen (DO), and pH
Marine Science Research Facility Contact: Dawn Outram	Analyzer - Nutrient Lachat QuickChem 8500 Flow Injection Nutrient Auto Analyzer Measures dissolved nutrients in water, Ammonia, Phosphate, Nitrite, Nitrate, and Silicate
Marine Science Research Facility Contact: Dawn Outram	Fluorometer Satlantic Fluorescence Induction Relaxation Fluorometer (FIRE) Measures variable fluorescence in phytoplankton
Marine Science Research Facility Contact: Dawn Outram	Incubator Percival Incubator/Growth Chamber Temperature and light controlled incubator for environmental applications
Marine Science Research Facility Contact: Dawn Outram	Radiometer Biospherical Instruments Quantum Scalar Laboratory (QSL) Radiometer - PAR Light Meter Measures Photosynthetically Active Radiation (PAR)
Marine Science Research Facility Contact: Dawn Outram	Spectrometer Ocean Optics Jaz Spectrometer Optical sensing instrumentation that measures light wavelengths. Its unique features and expandable platform makes it uniquely suited for field applications, remote sensing, process flow and quality assurance.
Marine Science Research Facility Contact: Dawn Outram	Vacuum Manifold Vacuum Filter Manifold Allows for filtration of 5 ports simultaneously, 25 and 47 mm filter cups available
URI Sources, Transport, Exposure & Effects of PFAS (STEEP) Contact: Simon Vojta	Mass Spectrometer SCIEX X500R QTOF PLATFORM coupled to an EXION LC AC SYSTEM A tandem (MS/MS) mass spectrometer combining a quadrupole and a time-of-flight analyzer allows collecting high-resolution MS and MRM spectra. The instrument is suitable for both quantitative analysis as well as non-targeted approach and operates under SCIEX OS software. The collected data are evaluated using an independent processing station (SCIEX Alpha Workstation 2020) equipped with SCIEX OS-Q software
Marine Science Research Facility Contact: Dawn Outram	Aquatic Culture - Algae and Live Feed Room Various manufacturers K-walls, small rearing tanks for live feed, flowing seawater and freshwater
Marine Science Research Facility Contact: Dawn Outram	Aquatic Culture - Phytoplankton Culture Wheels Various manufacturers Motorized and water driven, clear plexiglass, ambient and experimental temperature control, can accommodate culture bottles of differing sizes (1-3L)
Marine Science Research Facility Contact: Dawn Outram	Aquatic System - Dechlorination System - Freshwater Various manufacturers 2 Freshwater dechlorination tanks
Marine Science Research Facility Contact: Dawn Outram	Workstation - Environmental Chamber EnvironAir Environmental Chamber 100 sq ft Temperature control, Two range 4-24 C, Two range -4-24 C (to simulate polar conditions), On generator back up and emergency monitoring systems. (4 Units)
Marine Science Research Facility Contact: Dawn Outram	Aquatic System - Flowing Seawater System Various manufacturers Raw-Ambient temperature and Filtered- Chilled, Heated, Ambient. UV treated, air supply, emergency monitoring systems
Marine Science Research Facility Contact: Dawn Outram	Biospecimen Banking - Freezer - Walk-in -20 C Walk-In Freezer 185 sq ft Long term storage, On generator back up and emergency monitoring systems
Marine Science Research Facility Contact: Dawn Outram	Photoperiod controller Various manufacturers Wattstopper controller(Blount), Various individual timers
Marine Science Research Facility Contact: Dawn Outram	Pier Access Access to Graduate School of Oceanography pier Access to pier for deployment, collection, testing. Water depths 18-15 feet to intertidal zone
Marine Science Research Facility Contact: Dawn Outram	Aquatic System - Laminar Flow Tank Laminar Flow Tank 1000 gal, Seawater or Freshwater, Multiple speeds with precision control. Experimental chamber visable from 5 sides
Marine Science Research Facility Contact: Dawn Outram	Aquatic System - Mesocosm Tank Various manufacturers 10, 5 ft deep outdoor mesocosms, with available raw ambient seawater flow
Marine Science Research Facility Contact: Dawn Outram	Worksation - Marine General Wet Lab Various manufacturers 3 Buildings, 7,000 sq ft indoor, and nearly unlimited outdoor space, Multiple insulated tank sizes, various sized aquaria, possible to perform simultaneous replicates
Marine Science Research Facility Contact: Dawn Outram	Workstation - Marine Pathology and Transgenics Laboratory and Systems Various manufacturers Ozone treated effluent and separate effluent drain
Marine Science Research Facility Contact: Dawn Outram	Fluorometer Turner Designs Trilogy Measures extracted or in vivo chlorophyll fluorescence of samples, Field-portable
Marine Science Research Facility Contact: Dawn Outram	Sensor - Oxygen Sensor PreSens Water phase analysis

Microbiology

Facility

Instrument/Service/Resource Unit

Contact: Paul Bertone, PhD

Training and Technical Assistance

Service
Advise investigators with experimental design for single cell analysis including implementation of technical plans as well as analysis of results. Advise on single cell genomics, RNA-seq, ChIP-seq and bioinformatic analysis. Provide support for grant submissions.

Facility

Instrument/Service/Resource Unit

Bryant Unistructure Science Spaces

Contact: Christopher Reid

Biological Safety Cabinet

LABGARD ES class II, type A2 Biological Safety Cabinet
Maintain sterility and contain infectious work

Bryant Unistructure Science Spaces

Contact: Christopher Reid

Workstation - Anaerobic Chamber

Microbiology International Whitley DG250 Anaerobic Workstation
Simulate anoxic environments for anaerobic work

Bryant Unistructure Science Spaces

Contact: Christopher Reid

Workstation - Anaerobic Chamber

MBRAUN Labstar Anaerobic Glovebox Workstation
simulate anoxic environments for anaerobic work

(Independent Nonprofit)

Facility

Instrument/Service/Resource Unit

Contact: Alexander Margulis, PhD

Microplate Reader - Fluorescence

Fluoroskan Ascent
Measures sample fluorescence Excitation wavelengths 320-700 nm, emission 360-700 nm

Rhode Island Consortium for Nanoscience and Nanotechnology

Contact: Alexander Margulis, PhD

PCR Thermal Cycler

Illumina Eco real-time PCR system
The Eco Real-Time PCR System offers life science researchers a full-featured real-time PCR system at an attractive price. Its features include:  Four-color multiplexing  High Resolution Melt (HRM)  Fast PCR cycling: 40-cycle PCR in 40 minutes  User-friendly, MIQE-compliant software.

Facility

Instrument/Service/Resource Unit

Contact: Dr. Irene Andreu, PhD

Sample Preparation - Automated pipettor

Opentrons OT-2
Automated sample preparation platform for staining well plates

Facility

Instrument/Service/Resource Unit

IDeA COBRE for CardioPulmonary Vascular Biology Core

Contact: Valeria Zarate

Imager and Microplate Reader

BioTek Cytation 5 microplate reader
Imaging plate reader; 5 colors (brightfield, GFP, RFP, DAPI, Cy5) 4x, 10x, 20x objectives.

Molecular & Cell Biology

Facility	Instrument/Service/Resource Unit
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Cell Counter Life Technologies Automated Countess Cell Counter Measure cell count and viability (live, dead, and total cells) using the standard trypan blue technique.
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Microarray Platform Affymetrix Gene Chip Instrument System A fully integrated platform for Affymetrix microarrays
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Microarray Scanner Molecular Devices GenePix Scanner 4000B Two color microarray scanner
Mouse Transgenic and Gene Targeting Facility Contact: Jinping Luo, PhD	Electroporation System ThermoFisher Scientific Neon Transfection System Nucleic acid and protein electroporation
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	Lentivirus-mediated Stable Gene Over-expression in Cells Service
Mouse Transgenic and Gene Targeting Facility Contact: Jinping Luo, PhD	Spectrophotometer ThermoFisher Scientific NanoDrop Lite Measures nucleic acid and protein concentration
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Bioanalyzer Agilent Bioanalyzer 2100 Microfluidics-based platform for sizing, quantification and quality control of DNA, RNA.
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Analyzer - Fragment Advanced Analytical, Fragment Analyzer Capillary Electrophoresis - Analyze DNA fragments for Next-Gen Sequencing, genotyping, checking RNA quality and quantity, and working with microsatellite simple sequence repeats (SSRs).
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Centrifuge Sorvall Legend T Benchtop Low to medium speed centrifugation
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Centrifuge - Ultra Beckman Coulter Optima Max UltraCentrifuge High speed centrifugation with MLA130, TLA110 & TLA100 Rotors
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Disrupter Covaris S220 High Performance Ultrasonicator DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Imager and Microplate Reader BioTek Cytation 5 Cell Imaging Multi-Mode Reader UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence. Cell migration and invasion assays, Live cell imaging, Fluorescence cell imaging (DAPI & GFP), Multi-mode reader applications, Immunofluorescence, Phenotypic assays, Histology
Drosophila Media Facility Contact: Katherine Patenaude	Drosophila Media Preparation Service The Drosophila Media Facility is dedicated to providing investigators with high quality media for Drosophila research. In the centralized kitchen, a standard cornmeal, sucrose, yeast medium is prepared and dispensed into different sized culture vessels. Media is prepared according to a strict protocol that ensures consistency in diet and the absence of any bacterial, fungal or insect contamination. Researchers using glass vials return them to the facility where they are autoclaved, washed, and filled again. This centralized kitchen is equipped with a food-service quality Cleveland Range Kettle MKET-12-T. Media is pumped into trays of vials or plastic bottles using a Gilson Fly Food Dispenser. Used glass vials are returned to the facility where they are sterilized in a Steris autoclave and washed in a Northwestern Systems G403 electric heat glassware washer.
Genomics Facility - Brown Contact: Christoph Schorl, PhD	PCR Thermal Cycler - Droplet Digital Bio-Rad QX200 Droplet Digital (ddPCR) PCR System Provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Genomics Facility - Brown Contact: Christoph Schorl, PhD	PCR Thermal Cycler Applied Biosystems ViiA 7 Real-Time PCR System Medium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput
Genomics Facility - Brown Contact: Christoph Schorl, PhD	PCR Thermal Cycler Applied Biosystems ViiA 7 Real-Time PCR System Medium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Spectrophotometer Thermo Scientific Nanodrop One A microvolume spectrophotometer for measuring DNA and RNA.
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Technical Assistance - Affmetrix Microarray Service Service to provide Affymetrix gene expression array service
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Technical Assistance - NGS Library Quality Control Service Analysis of library size distribution and qPCR quantification
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Analyzer - nCounter Systems NanoString - nCounter Sprint Profiler Molecular barcoding technology for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Included nSolver Analysis Software. Compatible with FFPE tissue. Highly multiplexed analysis of many functional pathways such as among others cancer, inflammation, cell death and custom designed expression analysis. Operated as fee for service.
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	Lentivirus-mediated CRISPR/Cas9 Gene Knock-out in Cells Service
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	Site-directed Mutagenesis Service
Lentivirus Construct Core (Legorreta Cancer Center at Brown University) Contact: Olin Liang, Ph.D.	Virus Packaging Service
Genomics Facility - Brown Contact: Christoph Schorl, PhD	Microplate Reader BioTek Synergy H1 Hybrid Multimode (H1MM) Microplate Reader UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence. Cell based assays, Biomarker assays, Micro-volume analysis of nucleic acid and protein, Kinetic assays.
Drug Discovery Core (Legorreta Cancer Center at Brown University) Contact: Shengliang Zhang, PhD	Training and Technical Assistance Service Advise interested investigators on cancer drug screens, cell-based assay development, assay optimization, and use of various chemical libraries/FDA-approved drugs to support research in the area of drug discovery and development. Advise on target validation, and interface with medicinal chemistry. Provide technical support for screening implementation, library plating, high-throughput robotic screening, and advice on prioritization of hits. Provide support for grant submissions.
Translational Oncology Core (Legorreta Cancer Center at Brown University) Contact: Attila Seyhan, PhD	Training and Technical Assistance Service Advise investigators on study design leading to translational clinical protocols. For prioritized studies, provide assistance with clinical protocol preparation as well as NIH, Industry or Foundation grant submissions to secure funding for the research.
CyTOF Core (Legorreta Cancer Center at Brown University) Contact: Patrycja Dubielecka-Szczerba, PhD	Training and Technical Assistance Service Advise investigators with experimental design, implementation and data analysis. Provide support for grant submissions.
Organic Synthesis Core (Legorreta Cancer Center at Brown University) Contact: Xiaobing Tian, PhD	Training and Technical Assistance Service Assist investigators with experimental plans for drug synthesis for lead optimization. Facilitate organic synthesis efforts through interactions with the Department of Chemistry, Industry or other collaborations. Provide support for grant submissions.
Organoids Core (Legorreta Cancer Center at Brown University) Contact: Lanlan Zhou, MD, PhD	Training and Technical Assistance Service Advise and assist investigators with the production of organoids to facilitate cancer biology, drug efficacy and toxicity evaluation of therapeutics towards tumor versus normal organoids that express different cancer gene mutants. Provide support for the growth and maintenance of organoid and spheroid cultures including passaging. Maintain a collection of established organoids that can be provided to investigators for research. Develop gene knockout and overexpression matched patient-derived organoids. Assist investigators with planning of drug screens working with the drug discovery capability. Provide support for grant submissions.

Facility	Instrument/Service/Resource Unit
Bryant Unistructure Science Spaces Contact: Christopher Reid	PCR Thermal Cycler Bio-Rad PCR Thermocycler C1000 Touch DNA amplifier
Bryant Unistructure Science Spaces Contact: Christopher Reid	Spectrophotometer Thermo Scientific AquaMate Plus UV/Vis spectrophotometer for quantifying concentrations
Bryant Unistructure Science Spaces Contact: Christopher Reid	Spectrophotometer Shimazdzu HPLC--equipped with UV/Vis and Evaporative light scattering detectors UV/Vis spectrophotometer for quantifying concentrations
Bryant Unistructure Science Spaces Contact: Christopher Reid	Biospecimen Banking - Freezer Thermo Scientific Isotemp Basic U86-13A41 minus 70 degree freezer Long term storage of biological specimens
Bryant Unistructure Science Spaces Contact: Christopher Reid	Sample Preparation - Freeze Dry System Labconco FreeZone 4.5 Freeze dryer
Bryant Unistructure Science Spaces Contact: Christopher Reid	PCR Thermal Cycler Bio-Rad CFX96 touch RT-PCR This real-time pcr instrument allows for up to 5-target multiplexing in sample volumes as small as 10ul. It also has a thermal gradient feature in a 96-well format.

Facility	Instrument/Service/Resource Unit
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Imaging System - Gel Imager Bio-Rad Chemidoc XRS The ChemiDoc XRS+ system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Imaging System - Gel Imager Bio-Rad Molecular Imager FX system Imager with 50 micron resolution and a 5 order of magnitude linear dynamic range for fluorescence and radioisotopic imaging. You can also image and accurately quantitate chemiluminescent samples.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Cell Sorter - Magnetic-Activated Cell Sorter (MACS) Miltenyi AutoMACS Magnetic cell sorter Automated cell sorter, for separating cell suspensions labeled with magnetic beads
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microplate Reader Molecular Devices Spectramax Gemini EM Fluorescence Microplate Reader Enables top and bottom reading of microplates over a greater range of fluorescence intensities all without the use of filters.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Microplate Reader Perkin Elmer TopCount NXT Microplate Scintillation and Luminescence Counter TopCount NXT analyzes two, six, or twelve samples at a time, and counts samples in 24-, 96-, and 384-well microplates, as well as microcentrifuge tubes. It can count gammas, betas, or luminescent samples.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	PCR Thermal Cycler Applied Biosystems ABI 7500 96-well quantitative PCR
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Spectrophotometer Bio-Rad SmartSpec 90-260 V, UV/visible benchtop spectrophotometer for nucleic acid and protein quantitation, wavelength range 200–800 nm, built-in thermal printer
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Spectrophotometer Thermo Scientific NanoDrop 2000 A full spectral microvolume UV-Vis analysis spectrophotometer for measuring DNA, RNA, and protein.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Suspension Array System Bio-Rad BioPlex 200 Detection and quantification of multiple analytes (proteins and peptides, or nucleic acids) in a single sample volume.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Analyzer - Nanoparticle counter Malvern Nanosight NS300 Analysis of size distribution and concentration of nanoparticles from 10nm to 2000nm in diameter. A 532nm green laser also allows the detection of red fluorescent particles. A syringe pump allows sample analysis under constant flow, increasing reliability and reproducibility of data.
Molecular Biology and Imaging Core Contact: Sunil Shaw, PhD	Single Cell Analysis for RNAseq 1Cell Bio InDrop The InDrop enables single cell encapsulation, barcoading of cDNA, preparation of a sequencing librrary, and informatics.

Facility

Instrument/Service/Resource Unit

Contact: John W. Morgan, Ph.D.

Molecular Core

Applied Biosystem PRISM 7000 Sequence Detection System, Bio-Rad ChemiDoc Molecular Imager, Bio-Rad CFX96 Touch Real-Time PCR Detection System
The molecular core offers the capability of performing PCR analysis, quantitative real-time PCR analysis, and digital gel documentation.

Facility	Instrument/Service/Resource Unit
CCRI Biology Labs Contact: David Vito	Autoclave Consolidated Stills & Sterilizers 24D36 Sterilization. Chamber size is 24inch x36inch, Volume 9.4 cu. Ft
CCRI Biology Labs Contact: David Vito	Balance - Analytical Orion Analytical Balance Weight determination with capacity of 60g.
CCRI Biology Labs Contact: David Vito	Balance - Top Loading EK-I Compact Balance Weight determination (2 units)
CCRI Biology Labs Contact: David Vito	Incubator Fisher Scientific Isotemp Digital & Analog Dry Bath Incubator Provide controlled dry heat environment for clinical & general chemistry applications
CCRI Biology Labs Contact: David Vito	Electrophoresis System Bio-Rad Mini-Protean Tetra Cell, Vertical protein electrophoresis gel boxes Running Protein or DNA polyacrylamide gels
CCRI Biology Labs Contact: David Vito	Microscope Lieder MI-500 Series Compound Inverted Microscope Tissue Culture
CCRI Biology Labs Contact: David Vito	pH Meter Thermo Scientific Orion 2-Star Bench Top pH Meter General purpose measurements
CCRI Biology Labs Contact: David Vito	Western Blotting System, Semi-Dry Shelton Scientific Semi-Dry Blotting System Transfer of protein, RNA & DNA gels to a solid membrane support for visualization, 24cmx30cm.
CCRI Biology Labs Contact: David Vito	Spectrophotometer Thermo Scientific Genesis 20 Visible Spectrophotometer UV spectrophotometer for quantifying concentrations
CCRI Biology Labs Contact: David Vito	Imaging System - Gel Imager - Transilluminator FOTODYNE Foto Phoresis UV Transilluminator Imaging-protein gel analysis, GFP Analysis
CCRI Biology Labs Contact: David Vito	Incubator - Shaker Lab Companion IS-971 Floor Model Incubator Shaker Growing of bacteria, 250 L chamber
CCRI Biology Labs Contact: David Vito	Biological Safety Cabinet NuAire Class II Type A2 Maintain sterility and contain infectious work

(Independent Nonprofit)

Facility

Instrument/Service/Resource Unit

Rhode Island Nuclear Science Center

Contact: Alexander Margulis, PhD

Chemical Hood

Labconco Protector Premier Fume Hood
Suitable for providing personal protection needed for general chemistry applications involving fumes and vapors.

Facility	Instrument/Service/Resource Unit
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Incubator - Shaker Lab Companion SI-600 Incubated Shaker Bacterial culture shaker with a temperature of ambient to +60C.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	PCR Thermal Cycler Bioer Life ECO thermal cycler This thermal cycler has a 96 well format with a 2C/sec ramp rate.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	High Performance Liquid Chromatography (HPLC) Agilent 1290 Infinity II Modular UHPLC system with automated multisampler and quaternary pump for analyte separations up to 600 bar.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	High Performance Liquid Chromatography (HPLC) Thermo Scientific nLC 1200 High-performance LC for nanoflow resolution of complex analyte mixtures at up to 1200 bar.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Cell Counter Thermo Scientific Countess II Automated Cell Counter Automated image analysis of cell suspensions providing rapid measurement of total cell counts, viability and average cell size.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Spectrophotometer Thermo Scientific NanoDrop 2000 Measures DNA, RNA, and protein concentrations, and purity (A260/A280 ratio). Conc. range= 2 ng/microliter-15,000 ng/microliter; Min Sample size = 0.5 microliter.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	PCR Thermal Cycler Applied Biosystems ProFlex thermal cycler This thermal cycler has a 3x32 well format that allows for 3 separate programs to be run. The ramp rate is 6C/sec.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	PCR Thermal Cycler Applied Biosystems Veriti thermal cycler This thermal cycler has a 96 well format with 3.9C/sec ramp rate.
Extracellular Vesicle Core Contact: Sicheng Wen MD, PhD, Director	PCR Thermal Cycler ViiA 7 real-time PCR system with twister robot We identify extracellular vesicles MicroRNA/mRNA profiling using the ABI TaqMan PCR array by ViiA 7 Real-Time PCR System, which is fully compatible with any standard or fast-cycling 384- or 96-well plates and TaqMan Array Micro Fluidic Cards and allows quickly and efficiently look up to 348 unique microRNAs/mRNAs per sample with as little as 1ng of input total RNA with pre-amplification.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	PCR Thermal Cycler MJ Opticon 2 real-time PCR system. This 96-well system accepts low-profile tubes or plates. There are two fluorophore channels with emission spectra in the 523-543 nm range and 540-700nm.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Incubator (4) NuAire IR water-jacketed CO2 incubators (NU-8500) This incubator provides an optimal in-vitro growth environment for both cell and tissue culture while minimizing dessication through a closed loop HEPA filtration system.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Biological Safety Cabinet (4) NuAire Bio Safety Cabinets (Class II, Type A/B3) These biological safety cabinets reduce exposure to airborne biological or particulate chemical agents in low to moderate risk-hazard research and/or drug preparations.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Centrifuge Sorvall Legend X1R centrifuge Process samples for diverse laboratory applications with rotors for 1.5ml eppendorf tubes, 15, and 50ml conical tubes. Refrigerated with a max speed of 152000 rpm.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Autoclave Amerex HVE-50 top loading autoclave sterilizer This is a 50L, self-contained, portable, top-loading autoclave with auto-exhaust and warming cycles.
Extracellular Vesicle Core Contact: Sicheng Wen MD, PhD, Director	Analyzer - Nanoparticle Counter NanoSight 5000 Nanoparticle Counter Nanoparticle tracking analysis (NTA) – direct and real-time visualization and analysis of nanoparticles in liquids, and calculate the size, size distribution and concentration of the particles. Brownian motion of nanoparticles is analyzed using the Stokes-Einstein equation.
Extracellular Vesicle Core Contact: Sicheng Wen MD, PhD, Director	Ultrafiltration TangenX By using tangential flow filtration (TFF) technology, TangenX delivers rapid and highly efficient isolation of extracellular vesicles from large volumes of samples.
Extracellular Vesicle Core Contact: Sicheng Wen MD, PhD, Director	Centrifuge - Ultra Thermo Fisher WX80 ultracentrifuge Ultracentrifugation is the classical method for extracellular vesicles isolation. The Extracellular Vesicle Core contains two Thermo Fisher WX80 ultracentrifuges with Sorvall AH-629 rotors.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Luminometer Promega Glomax 20/20 luminometer with printer Ultrasensitive luminescence detection in a single microcentrifuge tube (1.5ml) format.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Oven - Hybridization Oven Fisher Scientific Isotemp hybridization chamber Temperature range of +7C to 85C. Includes a rotisserie.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Microplate Reader Molecular Devices SpectraMax M2e plate reader Features top and bottom read capability for absorbance, fluoresence, luminescence, and time-resolved fluorescence.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Bioanalyzer Invitrogen Luminex 200 with xMap technology This is a versatile, efficient standard in multiplexing with the ability to use magnetic or polystyrene beads on up to 100 targets/sample.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	pH Meter Orion pH meter 920 pH range of -2 to 19.99 and oxygen range of -2 to 19.99ppm. Autocalibration buffers of 4.01, 7, and 10.01.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	pH meter Hanna Instruments 2209 This pH meter features manual pH calibration and temperature compensation.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Incubator GQF 1502 Sportsman egg incubator This is a complete incubator and hatching system with 3 automatically turned racks. It has an accurate digital thermostat with LCD of humidity and temperature.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Homogenizer Fisher Scientific, Powergen 125 Tissue Homogenizer Process volumes from 0.03 to 100mL. Variable speed control to 30,000rpm for efficient cell disruption. Disposable plastic generators eliminate sample cross-contamination. Flat-bottom stainless-steel generators process liquid or soft tissues.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Disrupter Qsonica 700W sonicator with 5.5" cup horn This sonicator has full amplitude control from 1-100%.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	PCR Thermal Cycler - Digital RainDrop Digital PCR by RainDance The RainDrop is composed of a Source instrument that can generate 10million droplets/sample and the Sense instrument, which can detect whether the PCR reactions are positive or negative for your fluorophore. Multiplexing is possible for the 8 samples that are run at a time on the microfluidics chip.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	PCR Thermal Cycler Bio-Rad CFX96 Real-Time PCR system This real-time PCR instrument allows for up to 5-target multiplexing in sample volumes as small as 10ul. It also has a thermal gradient feature in a 96-well format.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Spectrophotometer Thermo Scientific NanoDrop 2000c This is a full spectrum, UV-Vis spectrophotometer used to quantify and assess the purity of DNA, RNA, protein, and more at sample volumes as low as 0.5ul.
Imaging and Molecular Biology and Nanomedicine Core Contact: Lei Wei, PhD	Bioreactor Flexcell FX5000 Tension System This is a computer-regulated bioreactor that uses vacuum pressure to apply either cyclic or static strain to cells cultured on flexible-bottomed culture plates.

Facility	Instrument/Service/Resource Unit
Animal Models Core Contact: Gerard Nau, MD	Biospecimen Banking - Freezer - -80 degrees PHCbi PHCbi TwinGuard Series upright -80°C freezer for sample storage. Two independent refrigeration systems provides redundancy for sample protection.
Animal Models Core Contact: Gerard Nau, MD	PCR Thermal Cycler - Real-Time Detection System Bio-Rad CFX Connect Real-Time PCR Detection System CFX Connect Real-Time PCR System for two-target multiplex PCR and routine detection of single fluorophore experiments.
Animal Models Core Contact: Gerard Nau, MD	Microplate Reader Molecular Devices Spectramax i3X The Spectramax i3X has the ability to measure absorbance, fluorescence, and luminescence. Additional features include fluorescence polarization and time-resolved fluorescence, along with injectors that facilitate flash based applications including dual luciferase and ATP assays.
Animal Models Core Contact: Gerard Nau, MD	PCR Thermal Cycler - Droplet Digital Bio-Rad QX200 Droplet Digital PCR System Absolute quantification of target DNA or RNA molecules using EvaGreen or probe-based digital PCR applications. Flexible platform in groups of 8 with no standard curves necessary.
Animal Models Core Contact: Gerard Nau, MD	Neutralization assay Service Luciferase-based assay used to measure the neutralization properties of serum, plasma, or monoclonal antibodies against SARS-CoV-2 based on a pseudotyped lentivirus.

Facility	Instrument/Service/Resource Unit
PC - Albertus Magnus Hall Labs Contact: Charles Toth	Microtome Porter-Blum MT-2B Ultramicrotome Porter-Blum MT-2B UltraMicrotomes, 2 Units
PC - Hickey Hall Labs Contact: Charles Toth	PCR Thermal Cycler Stratagene Mx3000p Real-time PCR Thermal Cycler This instrument is available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilent's qPCR software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation.
PC - Hickey Hall Labs Contact: Charles Toth	Cold Room Walk-In Cold Room
PC - Hickey Hall Labs Contact: Charles Toth	Analyzer - Cell Metabolism Seahorse Biosciencies, XFe Extracellular Flux Analyzers, XFe96 Platform for metabolic assays that simultaneously measures the two major energy producing pathways of the cell - mitochondrial respiration and glycolysis - in a microplate, in real-time.
PC - Hickey Hall Labs Contact: Charles Toth	Laboratory - Tissue Culture Tissue Culture Laboratory Space and instrumentation for tissue culture preparation
PC - Hickey Hall Labs Contact: Charles Toth	Imaging System - Gel Imager LI-COR Odyssey IR (Infrared) Imaging System Instrumentation for quantitating western blots.
PC - Hickey Hall Labs Contact: Charles Toth	Fluorescence Activated Cell Sorter (FACS) Cellometer Vision Image Cytometry The Vision CBA System combines the simplicity of image cytometry with the power of flow analysis software to offer user-friendly cell-based assays
PC - Hickey Hall Labs Contact: Charles Toth	Spectrophotometer Thermo Scientific NanoDrop UV/VIS

Facility	Instrument/Service/Resource Unit
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Autoclave Autoclave Microbiology/Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Biological Safety Cabinet Labconco class II Biological Safety Cabinet Used for tissue culture
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Biological Safety Cabinet Sterile laminar flow hood Microbiology prep
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Centrifuge Eppendorf 5810R Refrigerated Centrifuge Used for tissue culture
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Centrifuge Sorvall Discovery M120SE Mini Ultracentrifuge Research/Cell fractionation
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Centrifuge Sorvall Superspeed RC2 Centrifuge General use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Centrifuge Sorvall Superspeed RC5 Plus Centrifuge Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Centrifuge Thermo IEC Centra CL3R Refrigerated Centrifuge Genetics and Cell & Molec courses
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Cold Room Walk-In Cold Room Large cold room for experimentation and storage
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Dishwasher Dishwasher Microbiology/Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Disrupter French Pressure Cell Press Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Disrupter Polytron Tissue Grinder Slide/Sample Preparation
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Disrupter Sonicator General use and research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Disrupter Sonicator with cup Research and graduate courses
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Electroporation System Bio-Rad Micropulser Electroporator Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Sample Preparation - Freeze Dry System ATMO VAC 5009 freeze dryer Slide/Sample Preparation
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Biospecimen Banking - Freezer Thermolyne Liquid Nitrogen cryogenic storage system Cell storage
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Biospecimen Banking - Freezer Ultralow Freezer Used for freezing biological samples, 6 units
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Imaging System - Gel Imager InGenius Syngene Gel Documentation System Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Ice Machine Ice Machine General use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Imaging System - Dark Room Imaging System - Dark Room Gel imaging and analysis
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Incubator Non CO2 Incubator Used for tissue culture
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Incubator Thermo CO2 Incubators Used for tissue culture, 2 units
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Incubator Refrigerated Incubator Used for tissue culture
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Laboratory - Tissue Culture Tissue Culture Room A room outfitted with tissue culture instrumentation
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microplate Reader Lenovo Automated Plate Reader Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Microplate Reader Thermo Labsystems Multiskan MMCC/340 plate reader Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Sample Preparation - Oven Drying Oven General use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	PCR Thermal Cycler Bio-Rad IQS Real time PCR thermocycler Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	PCR Thermal Cycler Eppendorf Gradient Mastercycler PCR instrumentation, 2 units
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Incubator - Shaker Incubator/Shakers Multiple units
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Spectrophotometer BioRad SmartSpec Plus Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Spectrophotometer Eppendorf Biophotometer Gectrophotometerseral use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Spectrophotometer Spectronic 21 D UV/VIS Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Spectrophotometer Therm Spectronic Genesys UV/VIS Scanning Research, 6 units
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Spectrophotometer Thermo Scientific NanoDrop ND-1000 UV/VIS, 2 units
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Water Purification System Barnstead Still Water Purifier General use for teaching labs
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Water Purification System Millipore DirectQ (X2) Tissue culture
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Water Purification System Millipore NanoPure Diamond 2nd floor research use
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Imaging System - X-ray Film X-ray film processor Blot imaging
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Autoclave Autoclave Microbiology/Research
RIC - Fogarty Life Sciences Labs Contact: Dr. Rebeka Merson	Autoclave Autoclave Microbiology/Research

Facility	Instrument/Service/Resource Unit
Marine Science Research Facility Contact: Dawn Outram	Fluorometer Turner Designs 10-AU (Chlorophyll) Field Fluorometer Measures extracted or in vivo chlorophyll fluorescence of samples, Field-portable, can be set up for continuous-flow monitoring or discrete sample analyses
Marine Science Research Facility Contact: Dawn Outram	Incubator - Shaker Thermo Max-Q Orbital Shaker Table Orbital shaker with 18x24 in platform and variety of clamps
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Nuclear Magnetic Resonance (NMR) Spectrometer Bruker Avance III 300 with BBO probe Optimized for small molecule NMR
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Nuclear Magnetic Resonance (NMR) Spectrometer Varian 500 MHz NMR spectrometer Optimized for small molecule NMR
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Synthesizer, DNA/RNA MerMade-4 Oligonucleotide synthesizer DNA/RNA Synthesizer
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	Bioanalyzer Agilent Bioanalyzer 2100 Provides fast and automated separation, sizing and quantification of DNA, RNA and proteins by miniaturized Lab-on-a-Chip electrophoresis
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Centrifuge Beckman Avante High Speed Centrifuge High speed centrifuge
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Centrifuge Thermo MultiFuge Centrifuge table-top Centrifuge
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Centrifuge - Ultra Beckman Coulter Optima Beckman Optima L-100 XP ultracentrifuge Ultra centrifuge
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Centrifuge - Ultra Sorvall Micro Ultra Centrifuge Ultra centrifuge
Marine Science Research Facility Contact: Dawn Outram	Disrupter Biospec Mini Bead Beater Shakes tubes with beads to disrupt cells, DNA , RNA
Marine Science Research Facility Contact: Dawn Outram	Disrupter Branson Digital Ultasonic Liquid Sonifier Disrupts cells
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	Disrupter Covaris S220 High Performance Ultrasonicator Automated acoustic disruption and homogenization of cells and tissue samples using focused ultrasonic energy at 500 KHz. Performs DNA Shearing
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	DNA Sequencer Illumina MiSeq DNA NextGen Sequencing Performs clonal amplification, genomic DNA sequencing, and data analysis with base calling, alignment, variant calling, and reporting.
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	DNA Sequencer Applied Biosystems 3500xl Genetic Analyzer DNA Sanger Sequencing & Fragment Analysis: Fluorescence-based instruments utilizing capillary electrophoresis for DNA sequencing and fragment analysis.
Marine Science Research Facility Contact: Dawn Outram	Fluorometer Life Technologies Qubit Fluorometer Quantifies DNA, RNA, and protein
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	Fluorometer Invitrogen Qubit Fluorometer that uses fluorescent dyes bound to specific molecules to quantify DNA, RNA and proteins
Marine Science Research Facility Contact: Dawn Outram	Homogenizer Polytron Homogenizer PT-10-35-GT Breaks down plant and animal tissue for analysis, and performs homogenization, dispersion, mixing, and prepares emulsions
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	Sample Preparation - Library Preparation System IntegenX Apollo 324 System NextGen Sequencing (NGS) Library Preparation System Automated liquid handling instrument dedicated to the preparation of sample libraries for Next Generation Sequencing
Marine Science Research Facility Contact: Dawn Outram	Microplate Reader Molecular Devices Spectramax M5 Measures Fluorescence Intensity, Absorbance, Time Resolved Fluorescence, Fluorescence Polarization and Luminescence in cuvettes and multiple sized microplates.
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	PCR Thermal Cycler Applied Biosystems/Life Technologies ABI Vii7- qPCR Real Time-PCR
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	PCR Thermal Cycler Eppendorf Mastercycler PCR PCR
Marine Science Research Facility Contact: Dawn Outram	PCR Thermal Cycler Eppendorf Master Cycler Pro Replicates DNA
Marine Science Research Facility Contact: Dawn Outram	PCR Thermal Cycler Stratagene QPCR Mx3000P Analysis of gene expression, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Quantitative PCR Filters- FAM/Syber Green, Rox, Hex, CY3, CY5
Rhode Island INBRE Molecular Informatics Core (MIC) Contact: Christopher Hemme	PCR Thermal Cycler Roche LC480 qPCR Systems Quantitative PCR (qPCR) instrument. Used for highly accurate qualitative and quantitative detection of nucleic acids and gene expression.
Marine Science Research Facility Contact: Dawn Outram	Incubator - Shaker Thermo Max Q Incubated Shaker Heated incubated shaker
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Spectrophotometer Thermo Scientific NanoDrop UV/VIS
Marine Science Research Facility Contact: Dawn Outram	Spectrophotometer Thermo Scientific NanoDrop 1000 Microvolume UV-Vis spectrophotometer that quantifies DNA, RNA, and protein from small 1-2 microliter sample size
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Water Purification System - Deionized (DI) Millipore Milli-Q Water System DI Water System
Cell Analysis and Sorting Core Contact: Alan Rothman, M.D., Research Professor	Microplate Reader Perkin-Elmer Envision Multimodal plate reader- absorbance, luminescence, fluorescence, time-resolved fluorescence, AlphaScreen
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Incubator Heraeus Heracell Double Stacked Incubator The Heraeus Heracell Double Stacked Incubator has Copper Chambers, which protects samples while optimizing cell growth with fast recovery characteristics.
Rhode Island Consortium for Nanoscience and Nanotechnology Contact: Dr. Irene Andreu, PhD	Sample Preparation - Automated pipettor Opentrons OT-2 Automated sample preparation platform for staining well plates
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Mass Spectrometer ABSciex Qtrap 4500 Triple Quadrapole LC/MS Has Shimadzu HPLC system attached
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Microplate Reader Molecular Devices ID3 Microplate Reader Uv/vis, Chemilumenesence, fluorescence
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Spectrophotometer Genesys 50 UV/VIS UV/Vis
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Imager and Microplate Reader BioTek Cytation 5 Imager fluorescence imaging
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Analyzer - Metabolism Analysis Agilent Seahorse XFe96 Analyzer 96 well plate metabolism analysis

Facility	Instrument/Service/Resource Unit
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Microarray Scanner Illumina iScan Whole-genome genotyping, gene expression analysis, methylation analysis
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Autoclave Tuttnauer Equipment: consumable sterilization
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Bioanalyzer Agilent Bioanalyzer DNA size & quantification (quality check for downstream analyses, i.e. next gen sequencing)
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Analyzer - Genetic Applied Biosystems 3500 Genetic Analyzer Capillary Electrophoresis - Analyze fragment length polymorphisms (96 format) (2)
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Centrifuge Eppendorf Refrigerated Centrifuge Alcohol precipitation
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Centrifuge Eppendorf For centrifuging 96/384 plates
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Mass Spectrometer Sequenom high throughput SNP genotyping, epigenetics
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Microplate Reader BioTek DNA quantification
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Microplate Reader Fluidigm End Point Reader High throughput SNP genotyping
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	PCR Thermal Cycler Applied Biosystems 384 well DNA amplification for downstream analysis (i.e. sequenom processes) (2 units)
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	PCR Thermal Cycler Applied Biosystems 96 well DNA amplification for downstream analysis (i.e. fragment length polymorphisms)(2 units)
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Robotic Workstation - DNA Extraction Chemagen/Perkin Elmer Magnetic Separation Module DNA extraction from blood & saliva
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Robotic Workstation - Liquid Handler Sequenom Liquid Handler Transfers PCR chemistries to samples (96/384 format)
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Robotic Workstation - Liquid Handler Sequenom Nanodispenser Dispenses PCR product to Sequenom chip
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Robotic Workstation - Sample Preparation Iontorrent/Life Technologies Enrichment System Simplifies the workflows for the Ion PGM system by providing an automated solution for scalable and reproducible template preparation.
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Robotic Workstation - Sample Processing Thermo Scientific Cap-it-All Caps tubes to the optimal torque to maintain sample integrity and provide a hands free method to capping and decapping tubes quickly and easily.
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Robotic Workstation -Liquid Handler Beckman Coulter Lab Automation Workstation Automated liquid handler
Genomics Laboratory - Providence VA Medical Center Contact: John McGeary, PhD	Spectrophotometer Thermo Scientific NanoDrop DNA quantification
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Channel Cycling Metabolic System OmniTech ElectronicsModel #80 AP4RAT AccuPacer 4 Channel Rat Treadmill and #75 MS8 8 For rodent cardiopulmonary exercise testing
IDeA COBRE for CardioPulmonary Vascular Biology Core Contact: Valeria Zarate	Spectrophotometer NanoDrop Measures the concentration of peptides, DNA and RNA, purified protein, nanoparticles, perform protein assays and optical density measurements

Nuclear Science

Facility

Instrument/Service/Resource Unit

Contact: Dr. Cameron Goodwin

Reactor, Research and Test

2 Mega-Watt, light water cooled, pool type reactor
Neutron and gamma irradiation and experimental set-up, as well as analysis

Proteomics

Facility	Instrument/Service/Resource Unit
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Mass Spectrometer Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer is a bench-top ESI LC-MS/MS system that combines quadrupole precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection.
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Analyzer - Calorimeter Microcal Isothermal Titration 200 (ITC) Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Analyzer - Calorimeter Microcal VP Differential Scanning (DSC) Measurement of heat changes that occur in biomolecules during controlled increase or decrease in temperature.
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Fluorometer - Spectrofluorometer Horiba Jobin Yvon FluoroMax-4 Fluorescence detection. A heated cell allows temperature studies.
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Spectrometer JASCO J-815 Circular Dichroism (CD) Spectrometer Protein folding studies, Protein conformational studies, DNA/RNA interactions, Enzyme kinetics, Purity testing of optically active substances, quantitative analysis of pharmaceuticals, natural products chemistry, biochemistry and macromolecules, rapid scanning (time resolved) experiments. Peltier device for temperature studies.
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Spectrophotometer Thermo Scientific NanoDrop 8000 A full spectral microvolume (microliter) UV-Vis analysis spectrophotometer for measuring DNA, RNA, and protein of 8 samples simultaneously.
Proteomics Facility - Brown Contact: Mandar T Naik, PhD	Technical Assistance Service Service to provide Proteomics Technal Assistance
IDeA COBRE Center for Addiction & Disease Risk Exacerbation (CADRE) Contact: Jennifer Tidey, Ph.D.	Analyzer - Automated Immunoassay System ProteinSimple - Ella Next Generation ELISA system The Ella mutiplex analyzer allows for multi-plexing of multiple biomarkers on the same cartridge. Rather than performing a separate ELISA for each biomarker with more sample volume to run in triplicate, the ELLA can multi-plex biomarkers in triplicate within a single well, increasing the amount of data that can be generated from clinical samples.

(Independent Nonprofit)

Facility

Instrument/Service/Resource Unit

Contact: Alexander Margulis, PhD

Mass Spectrometer

Agilent LC/MSD VL mass spectrometer
The Agilent 1100 Series LC/MSD offers you the sensitivity, selectivity, and depth of molecular information inherent in mass spectrometry—in a package that is rugged, reliable, and surprisingly easy to learn, operate, and maintain

Contact: Alexander Margulis, PhD

High Performance Liquid Chromatography (HPLC)

Agilent 1100 HPLC system

Facility	Instrument/Service/Resource Unit
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Bioinformatics Analysis - MASCOT Software: Matrix Science MASCOT Search Engine Software for MS data analysis through statistical evaluation of matches between observed and projected peptide fragments.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Bioinformatics Analysis - ProteoIQ Software: NuSep ProteoIQ Mass Spec Data Analysis Software for post-analysis of MASCOT results. Protein and peptide probabilities generated and protein relative quantitation is performed by spectral counting. Normalized and with standard deviations.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Liquid Chromatography - Mass Spectrometer (LC - MS) Thermo Scientific Q Exactive Plus Mass Spectrometer LC-MS/MS system combines high-performance quadrupole with high resolution, accurate-mass (HR/AM) Orbitrap for total proteome characterization. Max resolving power= 140,000; max scan speed= 12Hz, Intra-scan dynamic range > 5000:, Quadrupole mass filter.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Mass Spectrometry Service Service to provide Imaging Mass Spectrometry Data Analysis.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Protein Determination Service Service to provide Protein Determination (BCA assay).
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Protein Extraction Service Service to provide ReadyPrep Protein Extraction Kit (Total Protein and Sequential Extraction).
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Protein In-Gel Digestion Service Service to provide In-gel digestion LC/MS analysis and protein database search.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Protein In-Solution Digestion Service Service to provide In-solution digestion LC/MS analysis and protein database search.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Protein Quantification Service Service to provide Data interpretation and protein quantification.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Technical Assistance - Protein Sample Clean-up Service Service to provide ReadyPrep 2D Cleanup Kit.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Mass Spectrometer Thermo Scientific TSQ Quantiva Triple-quadrupole MS for identification and absolute quantification of biomarkers, target proteins/peptides with known post-translational modifications in selected mass ranges.
Proteomics Core - Rhode Island Hospital Contact: Paul Bertone, PhD	Microplate Reader GloMax Discover - Multimode Detection System Multi-mode detection instrument offering flexible use of filters and includes: high-performance, luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities

Facility	Instrument/Service/Resource Unit
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Chromatography Metrohm Ion Chromatography 940 Vario Ion Chromatography
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Electrophoresis System - Gel Electrophoresis Bio-Rad 2D Gel System Two Dimensional (2D) Gel Electrophoresis
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Liquid Chromatography GE AKTA Fast Protein Liquid Chromatograph (FPLC) FPLC
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Chromatography Teledyne-ISCO CombiFlash System Flash Chromatography
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Liquid Chromatography - Mass Spectrometer (LC - MS) AB SCIEX TripleTOF 4600 and Shimadzu Prominence UFLC sytem ESI-TOF MS
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Analyzer - Protein Quality Tycho NT.6 The Tycho NT.6 verifies protein quality by looking at the structural integrity (or foldedness) of a protein. Changes in the intrinsic fluorescence of the sample are measured as a thermal ramp is applied. Changes in the fluorescence signal indicate transitions in the folding state of the protein. The Tycho can be used to study the stability of protein samples in different buffers or at different pH values. The measurement is completed in three minutes and uses a capillary tube to hold the sample.
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	High Performance Liquid Chromatography (HPLC) Shimadzu Nexera-i HPLC system High Performance Liquid Chromatography
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Mass Spectrometer Shimadzu Axima MALDI-TOF MS/MS MALDI MS
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Mass Spectrometer Thermo iCAPQ-a ICP-MS Elemental Analyzer
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Spectropolarimeter Jasco J-1100 Circular Dichroism (CD) Spectropolarimeter CD
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Spectrometer Thermo Nicolet 380 Fourier Transformed Infrared Spectrometer (FT-IR) FT-IR
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Characterization - Surface Plasmon Resonance Biacore T200 SPR The Biacore T200 is a surface plasmon resonance instrument which can be used to characterize molecular interactions between biomolecules ranging in size from ions to viruses. The Biacore software provides a range of tools for kinetic analyses. These analyses can be performed using a multicycle approach (many samples against one ligand or when different ligands are immobilized), or alternatively, using fast, single cycle kinetics.
Rhode Island INBRE Centralized Research Core Facility (CRCF) Contact: Dr. Ang Cai	Western Blotting System Bio-Rad Turbo Trans Blot system Western Blotting

Repository

Facility	Instrument/Service/Resource Unit
BioBank, Rhode Island Contact: Garrett Sullivan	BioBank - Biospecimen Banking - Freezer - -80 degrees Thermo Scientific HERAfreezer 24 cu. Ft. -80 degree freezer (3) Biospecimen Storage at -80 degrees. Holds up to 50,000 1ml samples
BioBank, Rhode Island Contact: Garrett Sullivan	BioBank - Biospecimen Banking - Refrigerator LABREPCO FUTURA Platinum Series 52 cu. ft. glass door refrigerator (3) Biospecimen Storage Refrigerators at 4 degrees. 4 degree hinged glass door refrigerator
BioBank, Rhode Island Contact: Garrett Sullivan	BioBank - Robotic Workstation - Sample Preparation Qiagen QiaCube DNA, RNA or Protein Purification
BioBank, Rhode Island Contact: Garrett Sullivan	Bioinformatics Analysis - Freezerworks Software: Freezerworks 2019 Ver 11.01.00 Sample management software
BioBank, Rhode Island Contact: Garrett Sullivan	BioBank - Biospecimen Banking Service Service to store biospecimens at -80 degrees, 4 degrees and in liquid nitrogen. All equipment is connected to back-up generator power and monitored 24/7 by a Rees alarm system.
BioBank, Rhode Island Contact: Garrett Sullivan	BioBank - Biospecimen Banking - Freezer - Liquid Nitrogen Taylor Wharton LABS 40K Liquid Nitrogen Storage System (2) Liquid nitrogen biospecimen storage system. Holds up to 40,000 1ml samples
Molecular Pathology Core - Brown Contact: David Silverberg	Biospecimen Banking - Freezer Revco -80 degree C Freezer Temporary Sample storage

Facility

Instrument/Service/Resource Unit